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Voltage-dependent membrane currents of cultured human neurofibromatosis type 2 Schwann cells.
Glia. 1998 Nov; 24(3):313-22.GLIA

Abstract

Previous experimental observations indicate that inhibition of voltage-dependent K+ currents suppresses proliferation of normal Schwann cells. In the present study we tested the opposite relationship, i.e., whether Schwann cells from tumors with abnormally high rates of proliferation would have an increase in membrane K+ currents. Whole-cell membrane currents were studied in cultured cells from schwannomas of two neurofibromatosis type 2 (NF2) patients (n = 53), one patient with a sporadic schwannoma (n = 22), and two control subjects (n = 41). Five different types of voltage-dependent membrane currents were found in all of the Schwann cells tested. Membrane depolarization activated outward K+ and Cl- currents; quinidine was found to block the K+ current (IC50 approximately 1 microM), and NPPB reduced the Cl- current. Ba2+-sensitive inward rectifier K+ currents, fast Na+ currents, and a transient, inactivating K+ current were less frequently observed. On average, NF2 cells were found to have statistically significant higher membrane potential and larger non-inactivating K+ outward current as compared to controls. Electrophysiological parameters of Schwann cells from a sporadic schwannoma showed a tendency for larger outward currents; however, the difference did not reach statistical significance. Together the data support the suggestion of a possible link between K+ outward current and proliferation of Schwann cells.

Authors+Show Affiliations

Department of Physiology, University of Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9775982

Citation

Kamleiter, M, et al. "Voltage-dependent Membrane Currents of Cultured Human Neurofibromatosis Type 2 Schwann Cells." Glia, vol. 24, no. 3, 1998, pp. 313-22.
Kamleiter M, Hanemann CO, Kluwe L, et al. Voltage-dependent membrane currents of cultured human neurofibromatosis type 2 Schwann cells. Glia. 1998;24(3):313-22.
Kamleiter, M., Hanemann, C. O., Kluwe, L., Rosenbaum, C., Wosch, S., Mautner, V. F., Müller, H. W., & Grafe, P. (1998). Voltage-dependent membrane currents of cultured human neurofibromatosis type 2 Schwann cells. Glia, 24(3), 313-22.
Kamleiter M, et al. Voltage-dependent Membrane Currents of Cultured Human Neurofibromatosis Type 2 Schwann Cells. Glia. 1998;24(3):313-22. PubMed PMID: 9775982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Voltage-dependent membrane currents of cultured human neurofibromatosis type 2 Schwann cells. AU - Kamleiter,M, AU - Hanemann,C O, AU - Kluwe,L, AU - Rosenbaum,C, AU - Wosch,S, AU - Mautner,V F, AU - Müller,H W, AU - Grafe,P, PY - 1998/10/17/pubmed PY - 2000/6/20/medline PY - 1998/10/17/entrez SP - 313 EP - 22 JF - Glia JO - Glia VL - 24 IS - 3 N2 - Previous experimental observations indicate that inhibition of voltage-dependent K+ currents suppresses proliferation of normal Schwann cells. In the present study we tested the opposite relationship, i.e., whether Schwann cells from tumors with abnormally high rates of proliferation would have an increase in membrane K+ currents. Whole-cell membrane currents were studied in cultured cells from schwannomas of two neurofibromatosis type 2 (NF2) patients (n = 53), one patient with a sporadic schwannoma (n = 22), and two control subjects (n = 41). Five different types of voltage-dependent membrane currents were found in all of the Schwann cells tested. Membrane depolarization activated outward K+ and Cl- currents; quinidine was found to block the K+ current (IC50 approximately 1 microM), and NPPB reduced the Cl- current. Ba2+-sensitive inward rectifier K+ currents, fast Na+ currents, and a transient, inactivating K+ current were less frequently observed. On average, NF2 cells were found to have statistically significant higher membrane potential and larger non-inactivating K+ outward current as compared to controls. Electrophysiological parameters of Schwann cells from a sporadic schwannoma showed a tendency for larger outward currents; however, the difference did not reach statistical significance. Together the data support the suggestion of a possible link between K+ outward current and proliferation of Schwann cells. SN - 0894-1491 UR - https://www.unboundmedicine.com/medline/citation/9775982/Voltage_dependent_membrane_currents_of_cultured_human_neurofibromatosis_type_2_Schwann_cells_ L2 - http://www.diseaseinfosearch.org/result/5175 DB - PRIME DP - Unbound Medicine ER -