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Increased endothelin ETB contractile activity in cultured segments of human temporal artery.
Acta Physiol Scand. 1998 Sep; 164(1):21-7.AP

Abstract

Contractions induced by endothelin-1, endothelin-3 and the selective ETB agonist, sarafotoxin S6c, were studied in segments of human temporal artery. The results in fresh arteries were compared with those obtained after 1 or 4 days in organ culture, and with the specific ETA antagonist FR 139,317, the specific, mixed antagonist bosentan, or the specific ETB antagonist, BQ 788. Sarafotoxin S6c induced no contractile activity in fresh artery segments, but elicited marked contractions after culture. This contraction was only slightly inhibited by FR 139,317, but was abolished by BQ 788. Contractions induced by endothelin-1 were antagonized by FR 139,317 and bosentan, but not by BQ 788. Organ culture did not change the overall pattern, but all concentration-response curves were shifted leftwards. Contractions induced by endothelin-3 were abolished by all antagonists in fresh arteries, but some activity was restored after organ culture. Sensitivity to endothelin-3 was markedly increased. The results suggest a change in endothelin receptors during organ culture, resulting in a marked increase in contractile ETB activity, and possibly some increase in ETA activity. Such changes illustrate the complexity of endothelin responses in this vascular bed.

Authors+Show Affiliations

Department of Neurology, University Hospital of Trondheim, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9777021

Citation

White, L R., et al. "Increased Endothelin ETB Contractile Activity in Cultured Segments of Human Temporal Artery." Acta Physiologica Scandinavica, vol. 164, no. 1, 1998, pp. 21-7.
White LR, Leseth KH, Juul R, et al. Increased endothelin ETB contractile activity in cultured segments of human temporal artery. Acta Physiol Scand. 1998;164(1):21-7.
White, L. R., Leseth, K. H., Juul, R., Adner, M., Cappelen, J., Aasly, J., & Edvinsson, L. (1998). Increased endothelin ETB contractile activity in cultured segments of human temporal artery. Acta Physiologica Scandinavica, 164(1), 21-7.
White LR, et al. Increased Endothelin ETB Contractile Activity in Cultured Segments of Human Temporal Artery. Acta Physiol Scand. 1998;164(1):21-7. PubMed PMID: 9777021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased endothelin ETB contractile activity in cultured segments of human temporal artery. AU - White,L R, AU - Leseth,K H, AU - Juul,R, AU - Adner,M, AU - Cappelen,J, AU - Aasly,J, AU - Edvinsson,L, PY - 1998/10/20/pubmed PY - 1998/10/20/medline PY - 1998/10/20/entrez SP - 21 EP - 7 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 164 IS - 1 N2 - Contractions induced by endothelin-1, endothelin-3 and the selective ETB agonist, sarafotoxin S6c, were studied in segments of human temporal artery. The results in fresh arteries were compared with those obtained after 1 or 4 days in organ culture, and with the specific ETA antagonist FR 139,317, the specific, mixed antagonist bosentan, or the specific ETB antagonist, BQ 788. Sarafotoxin S6c induced no contractile activity in fresh artery segments, but elicited marked contractions after culture. This contraction was only slightly inhibited by FR 139,317, but was abolished by BQ 788. Contractions induced by endothelin-1 were antagonized by FR 139,317 and bosentan, but not by BQ 788. Organ culture did not change the overall pattern, but all concentration-response curves were shifted leftwards. Contractions induced by endothelin-3 were abolished by all antagonists in fresh arteries, but some activity was restored after organ culture. Sensitivity to endothelin-3 was markedly increased. The results suggest a change in endothelin receptors during organ culture, resulting in a marked increase in contractile ETB activity, and possibly some increase in ETA activity. Such changes illustrate the complexity of endothelin responses in this vascular bed. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/9777021/Increased_endothelin_ETB_contractile_activity_in_cultured_segments_of_human_temporal_artery_ L2 - https://doi.org/10.1046/j.1365-201X.1998.0399e.x DB - PRIME DP - Unbound Medicine ER -