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Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags.
Proc Natl Acad Sci U S A. 1998 Oct 27; 95(22):13330-5.PN

Abstract

A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs reflects the level and complexity of gene expression in the sampled tissue. To date, the majority of plant ESTs are from nonwoody plants such as Arabidopsis, Brassica, maize, and rice. Here, we present a large-scale production of ESTs from the wood-forming tissues of two poplars, Populus tremula L. x tremuloides Michx. and Populus trichocarpa 'Trichobel.' The 5,692 ESTs analyzed represented a total of 3,719 unique transcripts for the two cDNA libraries. Putative functions could be assigned to 2,245 of these transcripts that corresponded to 820 protein functions. Of specific interest to forest biotechnology are the 4% of ESTs involved in various processes of cell wall formation, such as lignin and cellulose synthesis, 5% similar to developmental regulators and members of known signal transduction pathways, and 2% involved in hormone biosynthesis. An additional 12% of the ESTs showed no significant similarity to any other DNA or protein sequences in existing databases. The absence of these sequences from public databases may indicate a specific role for these proteins in wood formation. The cDNA libraries and the accompanying database are valuable resources for forest research directed toward understanding the genetic control of wood formation and future endeavors to modify wood and fiber properties for industrial use.

Links

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Authors+Show Affiliations

Sterky F
Department of Biotechnology, Kungl Tekniska Högskolan, Royal Institute of Technology, SE-10044 Stockholm, Sweden.
Regan S
No affiliation info available
Karlsson J
No affiliation info available
Hertzberg M
No affiliation info available
Rohde A
No affiliation info available
Holmberg A
No affiliation info available
Amini B
No affiliation info available
Bhalerao R
No affiliation info available
Larsson M
No affiliation info available
Villarroel R
No affiliation info available
Van Montagu M
No affiliation info available
Sandberg G
No affiliation info available
Olsson O
No affiliation info available
Teeri TT
No affiliation info available
Boerjan W
No affiliation info available
Gustafsson P
No affiliation info available
Uhlén M
No affiliation info available
Sundberg B
No affiliation info available
Lundeberg J
No affiliation info available

MeSH

ArabidopsisBrassicaCloning, MolecularCrosses, GeneticDNA, ComplementaryEnzymesExpressed Sequence TagsGene ExpressionGene LibraryGenes, PlantMolecular Sequence DataOryzaPlant ProteinsTreesZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9789088

Citation

Sterky, F, et al. "Gene Discovery in the Wood-forming Tissues of Poplar: Analysis of 5, 692 Expressed Sequence Tags." Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 22, 1998, pp. 13330-5.
Sterky F, Regan S, Karlsson J, et al. Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags. Proc Natl Acad Sci U S A. 1998;95(22):13330-5.
Sterky, F., Regan, S., Karlsson, J., Hertzberg, M., Rohde, A., Holmberg, A., Amini, B., Bhalerao, R., Larsson, M., Villarroel, R., Van Montagu, M., Sandberg, G., Olsson, O., Teeri, T. T., Boerjan, W., Gustafsson, P., Uhlén, M., Sundberg, B., & Lundeberg, J. (1998). Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags. Proceedings of the National Academy of Sciences of the United States of America, 95(22), 13330-5.
Sterky F, et al. Gene Discovery in the Wood-forming Tissues of Poplar: Analysis of 5, 692 Expressed Sequence Tags. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13330-5. PubMed PMID: 9789088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags. AU - Sterky,F, AU - Regan,S, AU - Karlsson,J, AU - Hertzberg,M, AU - Rohde,A, AU - Holmberg,A, AU - Amini,B, AU - Bhalerao,R, AU - Larsson,M, AU - Villarroel,R, AU - Van Montagu,M, AU - Sandberg,G, AU - Olsson,O, AU - Teeri,T T, AU - Boerjan,W, AU - Gustafsson,P, AU - Uhlén,M, AU - Sundberg,B, AU - Lundeberg,J, PY - 1998/10/28/pubmed PY - 1998/10/28/medline PY - 1998/10/28/entrez SP - 13330 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 95 IS - 22 N2 - A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs reflects the level and complexity of gene expression in the sampled tissue. To date, the majority of plant ESTs are from nonwoody plants such as Arabidopsis, Brassica, maize, and rice. Here, we present a large-scale production of ESTs from the wood-forming tissues of two poplars, Populus tremula L. x tremuloides Michx. and Populus trichocarpa 'Trichobel.' The 5,692 ESTs analyzed represented a total of 3,719 unique transcripts for the two cDNA libraries. Putative functions could be assigned to 2,245 of these transcripts that corresponded to 820 protein functions. Of specific interest to forest biotechnology are the 4% of ESTs involved in various processes of cell wall formation, such as lignin and cellulose synthesis, 5% similar to developmental regulators and members of known signal transduction pathways, and 2% involved in hormone biosynthesis. An additional 12% of the ESTs showed no significant similarity to any other DNA or protein sequences in existing databases. The absence of these sequences from public databases may indicate a specific role for these proteins in wood formation. The cDNA libraries and the accompanying database are valuable resources for forest research directed toward understanding the genetic control of wood formation and future endeavors to modify wood and fiber properties for industrial use. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/9789088/Gene_discovery_in_the_wood_forming_tissues_of_poplar:_analysis_of_5_692_expressed_sequence_tags_ DB - PRIME DP - Unbound Medicine ER -