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Isoelectrofocusing phenotype and relative concentration of transcobalamin II isoproteins related to the codon 259 Arg/Pro polymorphism.
Biochem Biophys Res Commun. 1998 Oct 29; 251(3):769-74.BB

Abstract

We investigated transcobalamin II (TC) isoelectrofocusing (IEF) phenotype and codon 259 polymorphism, in Caco-2 and HT-29 cells and in blood drawn from 39 healthy Caucasians. Caco-2 cells expressed a single TC variant (259-Arg), while HT-29 cells expressed TC with either Arg or Pro at codon 259 and exhibited two isoproteins in IEF with urea, but only one in IEF without urea. Among the Caucasians, 7 subjects expressed the TC 259-Arg variant, 10 the 259-Pro variant, and 22 were heterozygous. The TC 259-Pro isoprotein issued from HT-29 cells and heterozygous caucasian sera, was, respectively, 2. 4-fold and 1.6-fold higher than the TC 259-Arg isoprotein. Apo-TC and vitamin B12 serum concentrations in 259-Pro homozygotes were, respectively, 1.7 and 1.4-fold higher than those in 259-Arg homozygotes (p<0.005 and p=0.05). In conclusion, the 259-Arg/Pro polymorphism yields two TC variants only titratable in denaturing conditions and affects the blood level of both Apo-TC and vitamin B12.

Authors+Show Affiliations

Laboratoire de Pathologie Cellulaire et Moléculaire en Nutrition, EP CNRS 616, Vandoeuvre les Nancy, Cedex, 54505, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9790985

Citation

Namour, F, et al. "Isoelectrofocusing Phenotype and Relative Concentration of Transcobalamin II Isoproteins Related to the Codon 259 Arg/Pro Polymorphism." Biochemical and Biophysical Research Communications, vol. 251, no. 3, 1998, pp. 769-74.
Namour F, Guy M, Aimone-Gastin I, et al. Isoelectrofocusing phenotype and relative concentration of transcobalamin II isoproteins related to the codon 259 Arg/Pro polymorphism. Biochem Biophys Res Commun. 1998;251(3):769-74.
Namour, F., Guy, M., Aimone-Gastin, I., de Nonancourt, M., Mrabet, N., & Guéant, J. L. (1998). Isoelectrofocusing phenotype and relative concentration of transcobalamin II isoproteins related to the codon 259 Arg/Pro polymorphism. Biochemical and Biophysical Research Communications, 251(3), 769-74.
Namour F, et al. Isoelectrofocusing Phenotype and Relative Concentration of Transcobalamin II Isoproteins Related to the Codon 259 Arg/Pro Polymorphism. Biochem Biophys Res Commun. 1998 Oct 29;251(3):769-74. PubMed PMID: 9790985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoelectrofocusing phenotype and relative concentration of transcobalamin II isoproteins related to the codon 259 Arg/Pro polymorphism. AU - Namour,F, AU - Guy,M, AU - Aimone-Gastin,I, AU - de Nonancourt,M, AU - Mrabet,N, AU - Guéant,J L, PY - 1998/10/29/pubmed PY - 1998/10/29/medline PY - 1998/10/29/entrez SP - 769 EP - 74 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 251 IS - 3 N2 - We investigated transcobalamin II (TC) isoelectrofocusing (IEF) phenotype and codon 259 polymorphism, in Caco-2 and HT-29 cells and in blood drawn from 39 healthy Caucasians. Caco-2 cells expressed a single TC variant (259-Arg), while HT-29 cells expressed TC with either Arg or Pro at codon 259 and exhibited two isoproteins in IEF with urea, but only one in IEF without urea. Among the Caucasians, 7 subjects expressed the TC 259-Arg variant, 10 the 259-Pro variant, and 22 were heterozygous. The TC 259-Pro isoprotein issued from HT-29 cells and heterozygous caucasian sera, was, respectively, 2. 4-fold and 1.6-fold higher than the TC 259-Arg isoprotein. Apo-TC and vitamin B12 serum concentrations in 259-Pro homozygotes were, respectively, 1.7 and 1.4-fold higher than those in 259-Arg homozygotes (p<0.005 and p=0.05). In conclusion, the 259-Arg/Pro polymorphism yields two TC variants only titratable in denaturing conditions and affects the blood level of both Apo-TC and vitamin B12. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9790985/Isoelectrofocusing_phenotype_and_relative_concentration_of_transcobalamin_II_isoproteins_related_to_the_codon_259_Arg/Pro_polymorphism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(98)99463-3 DB - PRIME DP - Unbound Medicine ER -