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Neutralization of different geographic strains of the hepatitis E virus with anti-hepatitis E virus-positive serum samples obtained from different sources.
Virology. 1998 Sep 30; 249(2):316-24.V

Abstract

A recently developed polymerase chain reaction (PCR)-based cell culture neutralization assay was used to investigate cross-neutralization of known hepatitis E virus (HEV) strains obtained from various HEV-endemic regions of the world with different anti-HEV-positive serum samples. Serum specimens obtained from cynomolgus macaques experimentally infected with strains from Burma, Mexico, or Pakistan cross-neutralized the infectivity of each strain as well as an isolate from Morocco. Serum samples obtained either from infected patients who reside in HEV-endemic regions of the world or from U.S. residents who became infected while traveling to such regions also neutralized all four strains. In contrast, antibodies obtained from rabbits immunized with full-length Burma strain ORF2 protein neutralized only the Burma and Pakistan strains, not the Mexico or Morocco strains. In addition, antibodies obtained from guinea pigs immunized with an N-terminal truncated Burma strain ORF2 protein neutralized each strain except the Morocco strain. These data strongly suggest that antibodies elicited during an HEV infection demonstrate broad HEV neutralizing activity, whereas antibodies elicited after immunization with recombinant Burma ORF2 protein demonstrate a more limited ability to neutralize various HEV strains obtained from different regions of the world endemic for the disease.

Authors+Show Affiliations

National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia, 30333, USA. ZTAO@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9791023

Citation

Meng, J, et al. "Neutralization of Different Geographic Strains of the Hepatitis E Virus With Anti-hepatitis E Virus-positive Serum Samples Obtained From Different Sources." Virology, vol. 249, no. 2, 1998, pp. 316-24.
Meng J, Pillot J, Dai X, et al. Neutralization of different geographic strains of the hepatitis E virus with anti-hepatitis E virus-positive serum samples obtained from different sources. Virology. 1998;249(2):316-24.
Meng, J., Pillot, J., Dai, X., Fields, H. A., & Khudyakov, Y. E. (1998). Neutralization of different geographic strains of the hepatitis E virus with anti-hepatitis E virus-positive serum samples obtained from different sources. Virology, 249(2), 316-24.
Meng J, et al. Neutralization of Different Geographic Strains of the Hepatitis E Virus With Anti-hepatitis E Virus-positive Serum Samples Obtained From Different Sources. Virology. 1998 Sep 30;249(2):316-24. PubMed PMID: 9791023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutralization of different geographic strains of the hepatitis E virus with anti-hepatitis E virus-positive serum samples obtained from different sources. AU - Meng,J, AU - Pillot,J, AU - Dai,X, AU - Fields,H A, AU - Khudyakov,Y E, PY - 1998/10/29/pubmed PY - 1998/10/29/medline PY - 1998/10/29/entrez SP - 316 EP - 24 JF - Virology JO - Virology VL - 249 IS - 2 N2 - A recently developed polymerase chain reaction (PCR)-based cell culture neutralization assay was used to investigate cross-neutralization of known hepatitis E virus (HEV) strains obtained from various HEV-endemic regions of the world with different anti-HEV-positive serum samples. Serum specimens obtained from cynomolgus macaques experimentally infected with strains from Burma, Mexico, or Pakistan cross-neutralized the infectivity of each strain as well as an isolate from Morocco. Serum samples obtained either from infected patients who reside in HEV-endemic regions of the world or from U.S. residents who became infected while traveling to such regions also neutralized all four strains. In contrast, antibodies obtained from rabbits immunized with full-length Burma strain ORF2 protein neutralized only the Burma and Pakistan strains, not the Mexico or Morocco strains. In addition, antibodies obtained from guinea pigs immunized with an N-terminal truncated Burma strain ORF2 protein neutralized each strain except the Morocco strain. These data strongly suggest that antibodies elicited during an HEV infection demonstrate broad HEV neutralizing activity, whereas antibodies elicited after immunization with recombinant Burma ORF2 protein demonstrate a more limited ability to neutralize various HEV strains obtained from different regions of the world endemic for the disease. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/9791023/Neutralization_of_different_geographic_strains_of_the_hepatitis_E_virus_with_anti_hepatitis_E_virus_positive_serum_samples_obtained_from_different_sources_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(98)99346-1 DB - PRIME DP - Unbound Medicine ER -