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Morphology of single ganglion cells in the glaucomatous primate retina.
Invest Ophthalmol Vis Sci. 1998 Nov; 39(12):2304-20.IO

Abstract

PURPOSE

To examine the degenerative effects that prolonged elevation of intraocular pressure (IOP), a risk factor commonly associated with glaucoma, has on the morphology of single ganglion cells in the primate retina.

METHODS

The monkey model of glaucoma was combined with intracellular staining techniques using an isolated retina preparation. Midget and parasol cells from normal and glaucomatous eyes were labeled intracellularly, and their axons, somas, and dendritic fields were compared using confocal microscopy.

RESULTS

In midget and parasol cells, the earliest signs of pressure-induced degeneration involved structural abnormalities associated with the dendritic arbor. Reductions in axon thickness appeared later, with changes in soma size occurring concomitantly or slightly later. Chronic elevation of IOP resulted in a significant decrease in the mean soma sizes of midget and parasol cells, but only parasol cells showed a significant reduction in dendritic field size and axon diameter. Comparisons of eyes with different levels of optic nerve damage, based on cup- disc ratio, showed that the axons and dendritic fields of parasol cells were significantly smaller at lower cup-disc ratios than were those of midget cells, suggesting a possible differential effect.

CONCLUSIONS

In glaucoma, retinal ganglion cells undergo a pattern of degeneration that originates with the dendritic arbor and ends with shrinkage of the cell soma. Although this pattern of degeneration implies early functional deficits and retinal ganglion cell atrophy that occurs earlier than previously thought, based on ganglion cell loss alone, it also suggests a window of opportunity for effective neuroprotection.

Authors+Show Affiliations

Department of Anatomy, Center for Clinical Neuroscience and Ophthalmology, Michigan State University, East Lansing 48824, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9804139

Citation

Weber, A J., et al. "Morphology of Single Ganglion Cells in the Glaucomatous Primate Retina." Investigative Ophthalmology & Visual Science, vol. 39, no. 12, 1998, pp. 2304-20.
Weber AJ, Kaufman PL, Hubbard WC. Morphology of single ganglion cells in the glaucomatous primate retina. Invest Ophthalmol Vis Sci. 1998;39(12):2304-20.
Weber, A. J., Kaufman, P. L., & Hubbard, W. C. (1998). Morphology of single ganglion cells in the glaucomatous primate retina. Investigative Ophthalmology & Visual Science, 39(12), 2304-20.
Weber AJ, Kaufman PL, Hubbard WC. Morphology of Single Ganglion Cells in the Glaucomatous Primate Retina. Invest Ophthalmol Vis Sci. 1998;39(12):2304-20. PubMed PMID: 9804139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphology of single ganglion cells in the glaucomatous primate retina. AU - Weber,A J, AU - Kaufman,P L, AU - Hubbard,W C, PY - 1998/11/6/pubmed PY - 1998/11/6/medline PY - 1998/11/6/entrez SP - 2304 EP - 20 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 39 IS - 12 N2 - PURPOSE: To examine the degenerative effects that prolonged elevation of intraocular pressure (IOP), a risk factor commonly associated with glaucoma, has on the morphology of single ganglion cells in the primate retina. METHODS: The monkey model of glaucoma was combined with intracellular staining techniques using an isolated retina preparation. Midget and parasol cells from normal and glaucomatous eyes were labeled intracellularly, and their axons, somas, and dendritic fields were compared using confocal microscopy. RESULTS: In midget and parasol cells, the earliest signs of pressure-induced degeneration involved structural abnormalities associated with the dendritic arbor. Reductions in axon thickness appeared later, with changes in soma size occurring concomitantly or slightly later. Chronic elevation of IOP resulted in a significant decrease in the mean soma sizes of midget and parasol cells, but only parasol cells showed a significant reduction in dendritic field size and axon diameter. Comparisons of eyes with different levels of optic nerve damage, based on cup- disc ratio, showed that the axons and dendritic fields of parasol cells were significantly smaller at lower cup-disc ratios than were those of midget cells, suggesting a possible differential effect. CONCLUSIONS: In glaucoma, retinal ganglion cells undergo a pattern of degeneration that originates with the dendritic arbor and ends with shrinkage of the cell soma. Although this pattern of degeneration implies early functional deficits and retinal ganglion cell atrophy that occurs earlier than previously thought, based on ganglion cell loss alone, it also suggests a window of opportunity for effective neuroprotection. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/9804139/Morphology_of_single_ganglion_cells_in_the_glaucomatous_primate_retina_ DB - PRIME DP - Unbound Medicine ER -