Tags

Type your tag names separated by a space and hit enter

Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA.
Am J Surg Pathol. 1998 Nov; 22(11):1417-22.AJ

Abstract

We report a case of peripheral primitive neuroectodermal tumor (pPNET), which belongs to the PNET/Ewing's sarcoma family, arising in the left ovary of a 29-year-old woman. Microscopically, the tumor was composed of solid nests and sheets of monotonous, primitive, small round cells with a few rosettes, making it difficult to distinguish from small cell carcinoma of the ovary. Immunohistochemically, the tumor cells showed intense cell-membranous immunoreactivity for MIC2 protein (CD99). A short-term cell culture and karyotypic analysis revealed the tumor to possess a balanced t(11;22)(q24;q12) chromosomal translocation that is highly specific for tumors of the PNET/Ewing's sarcoma family. In addition, EWS/FLI-1 chimeric mRNA that originated from the characteristic chromosomal translocation was detected by reverse transcription-polymerase chain reaction. These results confirmed the diagnostic validity of the present tumor being a pPNET, thus raising the possibility that in the past, pPNETs which have arisen in the ovary may have been mistakenly diagnosed as small cell carcinomas of the ovary.

Authors+Show Affiliations

Department of Pathology, National Kyushu Cancer Center, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

9808135

Citation

Kawauchi, S, et al. "Peripheral Primitive Neuroectodermal Tumor of the Ovary Confirmed By CD99 Immunostaining, Karyotypic Analysis, and RT-PCR for EWS/FLI-1 Chimeric MRNA." The American Journal of Surgical Pathology, vol. 22, no. 11, 1998, pp. 1417-22.
Kawauchi S, Fukuda T, Miyamoto S, et al. Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA. Am J Surg Pathol. 1998;22(11):1417-22.
Kawauchi, S., Fukuda, T., Miyamoto, S., Yoshioka, J., Shirahama, S., Saito, T., & Tsukamoto, N. (1998). Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA. The American Journal of Surgical Pathology, 22(11), 1417-22.
Kawauchi S, et al. Peripheral Primitive Neuroectodermal Tumor of the Ovary Confirmed By CD99 Immunostaining, Karyotypic Analysis, and RT-PCR for EWS/FLI-1 Chimeric MRNA. Am J Surg Pathol. 1998;22(11):1417-22. PubMed PMID: 9808135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA. AU - Kawauchi,S, AU - Fukuda,T, AU - Miyamoto,S, AU - Yoshioka,J, AU - Shirahama,S, AU - Saito,T, AU - Tsukamoto,N, PY - 1998/11/10/pubmed PY - 1998/11/10/medline PY - 1998/11/10/entrez SP - 1417 EP - 22 JF - The American journal of surgical pathology JO - Am J Surg Pathol VL - 22 IS - 11 N2 - We report a case of peripheral primitive neuroectodermal tumor (pPNET), which belongs to the PNET/Ewing's sarcoma family, arising in the left ovary of a 29-year-old woman. Microscopically, the tumor was composed of solid nests and sheets of monotonous, primitive, small round cells with a few rosettes, making it difficult to distinguish from small cell carcinoma of the ovary. Immunohistochemically, the tumor cells showed intense cell-membranous immunoreactivity for MIC2 protein (CD99). A short-term cell culture and karyotypic analysis revealed the tumor to possess a balanced t(11;22)(q24;q12) chromosomal translocation that is highly specific for tumors of the PNET/Ewing's sarcoma family. In addition, EWS/FLI-1 chimeric mRNA that originated from the characteristic chromosomal translocation was detected by reverse transcription-polymerase chain reaction. These results confirmed the diagnostic validity of the present tumor being a pPNET, thus raising the possibility that in the past, pPNETs which have arisen in the ovary may have been mistakenly diagnosed as small cell carcinomas of the ovary. SN - 0147-5185 UR - https://www.unboundmedicine.com/medline/citation/9808135/Peripheral_primitive_neuroectodermal_tumor_of_the_ovary_confirmed_by_CD99_immunostaining_karyotypic_analysis_and_RT_PCR_for_EWS/FLI_1_chimeric_mRNA_ L2 - https://doi.org/10.1097/00000478-199811000-00013 DB - PRIME DP - Unbound Medicine ER -