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The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli.
FEMS Microbiol Lett. 1998 Oct 15; 167(2):295-301.FM

Abstract

Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their E. coli counterparts (78 and 89% identity, respectively). In E. coli, the oxyR gene is a transcriptional activator that, under oxidizing conditions, induces expression of a set of oxidative defence genes. OxyR null mutants are, therefore, sensitive to hydrogen peroxide. Introduction of the E. carotovora oxyR gene into an E. coli oxyR mutant resulted in transformants that were hydrogen peroxide resistant, indicating that the Erwinia protein was functional in E. coli.

Authors+Show Affiliations

Cancer Research Center, University of Missouri, Columbia, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9809430

Citation

Calcutt, M J., et al. "The oxyR Gene From Erwinia Carotovora: Cloning, Sequence Analysis and Expression in Escherichia Coli." FEMS Microbiology Letters, vol. 167, no. 2, 1998, pp. 295-301.
Calcutt MJ, Lewis MS, Eisenstark A. The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli. FEMS Microbiol Lett. 1998;167(2):295-301.
Calcutt, M. J., Lewis, M. S., & Eisenstark, A. (1998). The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli. FEMS Microbiology Letters, 167(2), 295-301.
Calcutt MJ, Lewis MS, Eisenstark A. The oxyR Gene From Erwinia Carotovora: Cloning, Sequence Analysis and Expression in Escherichia Coli. FEMS Microbiol Lett. 1998 Oct 15;167(2):295-301. PubMed PMID: 9809430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli. AU - Calcutt,M J, AU - Lewis,M S, AU - Eisenstark,A, PY - 1998/11/11/pubmed PY - 1998/11/11/medline PY - 1998/11/11/entrez SP - 295 EP - 301 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 167 IS - 2 N2 - Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their E. coli counterparts (78 and 89% identity, respectively). In E. coli, the oxyR gene is a transcriptional activator that, under oxidizing conditions, induces expression of a set of oxidative defence genes. OxyR null mutants are, therefore, sensitive to hydrogen peroxide. Introduction of the E. carotovora oxyR gene into an E. coli oxyR mutant resulted in transformants that were hydrogen peroxide resistant, indicating that the Erwinia protein was functional in E. coli. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/9809430/The_oxyR_gene_from_Erwinia_carotovora:_cloning_sequence_analysis_and_expression_in_Escherichia_coli_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.1998.tb13242.x DB - PRIME DP - Unbound Medicine ER -