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Effects of lipid mediators on the synthesis of leukaemia inhibitory factor and interleukin 6 by human bone marrow stromal cells.
Cytokine. 1998 Oct; 10(10):781-5.C

Abstract

Human bone marrow stromal cells regulate haematopoiesis by releasing cytokines such as leukaemia inhibitory factor (LIF) and interleukin 6 (IL-6). We have investigated the effects of 5 lipid mediators (i.e. 12-HETE, 15-HETE, platelet-activating factor (PAF), LTB4 and prostaglandin E2 (PGE2)) on their LIF and IL-6 synthesis. 12-HETE, 15-HETE and LTB4 (both at 1 microM) stimulate the LIF production by human bone marrow stromal cells grown in 10% fetal calf serum (FCS). In contrast, PAF and PGE2 have no effect. 12-HETE (1 microM) enhances LIF synthesis in serum free medium 7.7-fold and stimulates IL-1 induced LIF production. 12-HETE, 15-HETE, PAF and LTB4 have no effect on the spontaneous, serum- and cytokine-induced IL-6 synthesis by bone marrow stromal cells. In contrast PGE2 significantly stimulates serum-induced IL-6 synthesis. This study reports for the first time that lipid mediators may act on human haematopoiesis by modulating LIF and IL-6 synthesis by bone marrow stromal cells. Particularly, 12-HETE enhances LIF but not IL-6 synthesis. The different regulation of IL-6 and LIF synthesis in response to lipid mediators highlights the complexity of the cytokine regulation into the human bone marrow.

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Authors+Show Affiliations

Denizot Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr. Marcland, 87025 Limoges, France.
Trimoreau F
No affiliation info available
Praloran V
No affiliation info available

MeSH

12-Hydroxy-5,8,10,14-eicosatetraenoic AcidBone Marrow CellsCells, CulturedCulture Media, Serum-FreeDinoprostoneEnzyme-Linked Immunosorbent AssayGrowth InhibitorsHumansHydroxyeicosatetraenoic AcidsInterleukin-1Interleukin-6Leukemia Inhibitory FactorLeukotriene B4LipidsLymphokinesPlatelet Activating FactorStromal CellsTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9811531

Citation

Denizot, Y, et al. "Effects of Lipid Mediators On the Synthesis of Leukaemia Inhibitory Factor and Interleukin 6 By Human Bone Marrow Stromal Cells." Cytokine, vol. 10, no. 10, 1998, pp. 781-5.
Denizot Y, Trimoreau F, Praloran V. Effects of lipid mediators on the synthesis of leukaemia inhibitory factor and interleukin 6 by human bone marrow stromal cells. Cytokine. 1998;10(10):781-5.
Denizot, Y., Trimoreau, F., & Praloran, V. (1998). Effects of lipid mediators on the synthesis of leukaemia inhibitory factor and interleukin 6 by human bone marrow stromal cells. Cytokine, 10(10), 781-5.
Denizot Y, Trimoreau F, Praloran V. Effects of Lipid Mediators On the Synthesis of Leukaemia Inhibitory Factor and Interleukin 6 By Human Bone Marrow Stromal Cells. Cytokine. 1998;10(10):781-5. PubMed PMID: 9811531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of lipid mediators on the synthesis of leukaemia inhibitory factor and interleukin 6 by human bone marrow stromal cells. AU - Denizot,Y, AU - Trimoreau,F, AU - Praloran,V, PY - 1998/11/13/pubmed PY - 1998/11/13/medline PY - 1998/11/13/entrez SP - 781 EP - 5 JF - Cytokine JO - Cytokine VL - 10 IS - 10 N2 - Human bone marrow stromal cells regulate haematopoiesis by releasing cytokines such as leukaemia inhibitory factor (LIF) and interleukin 6 (IL-6). We have investigated the effects of 5 lipid mediators (i.e. 12-HETE, 15-HETE, platelet-activating factor (PAF), LTB4 and prostaglandin E2 (PGE2)) on their LIF and IL-6 synthesis. 12-HETE, 15-HETE and LTB4 (both at 1 microM) stimulate the LIF production by human bone marrow stromal cells grown in 10% fetal calf serum (FCS). In contrast, PAF and PGE2 have no effect. 12-HETE (1 microM) enhances LIF synthesis in serum free medium 7.7-fold and stimulates IL-1 induced LIF production. 12-HETE, 15-HETE, PAF and LTB4 have no effect on the spontaneous, serum- and cytokine-induced IL-6 synthesis by bone marrow stromal cells. In contrast PGE2 significantly stimulates serum-induced IL-6 synthesis. This study reports for the first time that lipid mediators may act on human haematopoiesis by modulating LIF and IL-6 synthesis by bone marrow stromal cells. Particularly, 12-HETE enhances LIF but not IL-6 synthesis. The different regulation of IL-6 and LIF synthesis in response to lipid mediators highlights the complexity of the cytokine regulation into the human bone marrow. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/9811531/Effects_of_lipid_mediators_on_the_synthesis_of_leukaemia_inhibitory_factor_and_interleukin_6_by_human_bone_marrow_stromal_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(98)90360-X DB - PRIME DP - Unbound Medicine ER -