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Synthesis of medium-chain-length polyhydroxyalkanoates in arabidopsis thaliana using intermediates of peroxisomal fatty acid beta-oxidation.
Proc Natl Acad Sci U S A. 1998 Nov 10; 95(23):13397-402.PN

Abstract

Polyhydroxyalkanoate (PHA) is a family of polymers composed primarily of R-3-hydroxyalkanoic acids. These polymers have properties of biodegradable thermoplastics and elastomers. Medium-chain-length PHAs (MCL-PHAs) are synthesized in bacteria by using intermediates of the beta-oxidation of alkanoic acids. To assess the feasibility of producing MCL-PHAs in plants, Arabidopsis thaliana was transformed with the PhaC1 synthase from Pseudomonas aeruginosa modified for peroxisome targeting by addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. Immunocytochemistry demonstrated that the modified PHA synthase was appropriately targeted to leaf-type peroxisomes in light-grown plants and glyoxysomes in dark-grown plants. Plants expressing the PHA synthase accumulated electron-lucent inclusions in the glyoxysomes and leaf-type peroxisomes, as well as in the vacuole. These inclusions were similar to bacterial PHA inclusions. Analysis of plant extracts by GC and mass spectrometry demonstrated the presence of MCL-PHA in transgenic plants to approximately 4 mg per g of dry weight. The plant PHA contained saturated and unsaturated 3-hydroxyalkanoic acids ranging from six to 16 carbons with 41% of the monomers being 3-hydroxyoctanoic acid and 3-hydroxyoctenoic acid. These results indicate that the beta-oxidation of plant fatty acids can generate a broad range of R-3-hydroxyacyl-CoA intermediates that can be used to synthesize MCL-PHAs.

Authors+Show Affiliations

Institut de Biologie et Physiologie Vegetales, Batiment de Biologie, Universite de Lausanne, CH-1015 Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9811811

Citation

Mittendorf, V, et al. "Synthesis of Medium-chain-length Polyhydroxyalkanoates in Arabidopsis Thaliana Using Intermediates of Peroxisomal Fatty Acid Beta-oxidation." Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 23, 1998, pp. 13397-402.
Mittendorf V, Robertson EJ, Leech RM, et al. Synthesis of medium-chain-length polyhydroxyalkanoates in arabidopsis thaliana using intermediates of peroxisomal fatty acid beta-oxidation. Proc Natl Acad Sci U S A. 1998;95(23):13397-402.
Mittendorf, V., Robertson, E. J., Leech, R. M., Krüger, N., Steinbüchel, A., & Poirier, Y. (1998). Synthesis of medium-chain-length polyhydroxyalkanoates in arabidopsis thaliana using intermediates of peroxisomal fatty acid beta-oxidation. Proceedings of the National Academy of Sciences of the United States of America, 95(23), 13397-402.
Mittendorf V, et al. Synthesis of Medium-chain-length Polyhydroxyalkanoates in Arabidopsis Thaliana Using Intermediates of Peroxisomal Fatty Acid Beta-oxidation. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13397-402. PubMed PMID: 9811811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of medium-chain-length polyhydroxyalkanoates in arabidopsis thaliana using intermediates of peroxisomal fatty acid beta-oxidation. AU - Mittendorf,V, AU - Robertson,E J, AU - Leech,R M, AU - Krüger,N, AU - Steinbüchel,A, AU - Poirier,Y, PY - 1998/11/13/pubmed PY - 1998/11/13/medline PY - 1998/11/13/entrez SP - 13397 EP - 402 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 95 IS - 23 N2 - Polyhydroxyalkanoate (PHA) is a family of polymers composed primarily of R-3-hydroxyalkanoic acids. These polymers have properties of biodegradable thermoplastics and elastomers. Medium-chain-length PHAs (MCL-PHAs) are synthesized in bacteria by using intermediates of the beta-oxidation of alkanoic acids. To assess the feasibility of producing MCL-PHAs in plants, Arabidopsis thaliana was transformed with the PhaC1 synthase from Pseudomonas aeruginosa modified for peroxisome targeting by addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. Immunocytochemistry demonstrated that the modified PHA synthase was appropriately targeted to leaf-type peroxisomes in light-grown plants and glyoxysomes in dark-grown plants. Plants expressing the PHA synthase accumulated electron-lucent inclusions in the glyoxysomes and leaf-type peroxisomes, as well as in the vacuole. These inclusions were similar to bacterial PHA inclusions. Analysis of plant extracts by GC and mass spectrometry demonstrated the presence of MCL-PHA in transgenic plants to approximately 4 mg per g of dry weight. The plant PHA contained saturated and unsaturated 3-hydroxyalkanoic acids ranging from six to 16 carbons with 41% of the monomers being 3-hydroxyoctanoic acid and 3-hydroxyoctenoic acid. These results indicate that the beta-oxidation of plant fatty acids can generate a broad range of R-3-hydroxyacyl-CoA intermediates that can be used to synthesize MCL-PHAs. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/9811811/Synthesis_of_medium_chain_length_polyhydroxyalkanoates_in_arabidopsis_thaliana_using_intermediates_of_peroxisomal_fatty_acid_beta_oxidation_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=9811811 DB - PRIME DP - Unbound Medicine ER -
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