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Inter-alpha-inhibitor: a protein family involved in the inhibition of calcium oxalate crystallization.
Scanning Microsc. 1996; 10(2):425-33; discussion 433-4.SM

Abstract

Inter-alpha-inhibitor (I alpha I) is a serine protease inhibitor present in human plasma. It has a molecular weight of about 220 kDa which encompasses 3 chains including two heavy chains and one light chain. The light chain, known as bikunin, is responsible for the antitryptic activity of I alpha I in the inhibition of various enzymes, such as trypsin and chymotrypsin. Under physiologic or certain pathologic circumstances, several macromolecules related to I alpha I appear in plasma and urine. However, the physiologic role of I alpha I remains unclear. As far as urolithiasis is concerned, two urinary macromolecules related to I alpha I have been isolated and shown to be potent inhibitors of calcium oxalate formation. One of these inhibitors, uronic-acid-rich protein (UAP), has been identified and well characterized. The sequence of the first 18 amino acid residues of UAP is identical with that of bikunin. Furthermore, the immunoreaction between UAP and I alpha I antibody using immunoblot analysis was positive. UAP isolated from the urine of stone formers exhibited less inhibitory activity towards calcium oxalate crystallization than that derived from the urine of healthy subjects. This suggests a structural abnormality of the inhibitor obtained from stone patients. The organic matrix extracted from kidney stones contained a protein antigenically related to I alpha I. We conclude that UAP is a member of I alpha I family taking part in inhibiting calcium oxalate crystallization, and modulating the formation of stones in the urinary tract.

Authors+Show Affiliations

Univ. Florida College of Medicine, Dept. Pathology and Laboratory Medicine, Gainesville 32610-0275, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9813621

Citation

Atmani, F, et al. "Inter-alpha-inhibitor: a Protein Family Involved in the Inhibition of Calcium Oxalate Crystallization." Scanning Microscopy, vol. 10, no. 2, 1996, pp. 425-33; discussion 433-4.
Atmani F, Mizon J, Khan SR. Inter-alpha-inhibitor: a protein family involved in the inhibition of calcium oxalate crystallization. Scanning Microsc. 1996;10(2):425-33; discussion 433-4.
Atmani, F., Mizon, J., & Khan, S. R. (1996). Inter-alpha-inhibitor: a protein family involved in the inhibition of calcium oxalate crystallization. Scanning Microscopy, 10(2), 425-33; discussion 433-4.
Atmani F, Mizon J, Khan SR. Inter-alpha-inhibitor: a Protein Family Involved in the Inhibition of Calcium Oxalate Crystallization. Scanning Microsc. 1996;10(2):425-33; discussion 433-4. PubMed PMID: 9813621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inter-alpha-inhibitor: a protein family involved in the inhibition of calcium oxalate crystallization. AU - Atmani,F, AU - Mizon,J, AU - Khan,S R, PY - 1996/1/1/pubmed PY - 1998/11/14/medline PY - 1996/1/1/entrez SP - 425-33; discussion 433-4 JF - Scanning microscopy JO - Scanning Microsc VL - 10 IS - 2 N2 - Inter-alpha-inhibitor (I alpha I) is a serine protease inhibitor present in human plasma. It has a molecular weight of about 220 kDa which encompasses 3 chains including two heavy chains and one light chain. The light chain, known as bikunin, is responsible for the antitryptic activity of I alpha I in the inhibition of various enzymes, such as trypsin and chymotrypsin. Under physiologic or certain pathologic circumstances, several macromolecules related to I alpha I appear in plasma and urine. However, the physiologic role of I alpha I remains unclear. As far as urolithiasis is concerned, two urinary macromolecules related to I alpha I have been isolated and shown to be potent inhibitors of calcium oxalate formation. One of these inhibitors, uronic-acid-rich protein (UAP), has been identified and well characterized. The sequence of the first 18 amino acid residues of UAP is identical with that of bikunin. Furthermore, the immunoreaction between UAP and I alpha I antibody using immunoblot analysis was positive. UAP isolated from the urine of stone formers exhibited less inhibitory activity towards calcium oxalate crystallization than that derived from the urine of healthy subjects. This suggests a structural abnormality of the inhibitor obtained from stone patients. The organic matrix extracted from kidney stones contained a protein antigenically related to I alpha I. We conclude that UAP is a member of I alpha I family taking part in inhibiting calcium oxalate crystallization, and modulating the formation of stones in the urinary tract. SN - 0891-7035 UR - https://www.unboundmedicine.com/medline/citation/9813621/Inter_alpha_inhibitor:_a_protein_family_involved_in_the_inhibition_of_calcium_oxalate_crystallization_ DB - PRIME DP - Unbound Medicine ER -