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Overproduction of selenocysteine tRNA in Chinese hamster ovary cells following transfection of the mouse tRNA[Ser]Sec gene.
RNA. 1998 Nov; 4(11):1436-43.RNA

Abstract

Selenocysteine insertion during selenoprotein biosynthesis begins with the aminoacylation of selenocysteine tRNA[ser]sec with serine, the conversion of the serine moiety to selenocysteine, and the recognition of specific UGA codons within the mRNA. Selenocysteine tRNA[ser]sec exists as two major forms, differing by methylation of the ribose portion of the nucleotide at the wobble position of the anticodon. The levels and relative distribution of these two forms of the tRNA are influenced by selenium in mammalian cells and tissues. We have generated Chinese hamster ovary cells that exhibit increased levels of tRNA[ser]sec following transfection of the mouse tRNA[ser]sec gene. The levels of selenocysteine tRNA[ser]sec in transfectants increased proportionally to the number of stably integrated copies of the tRNA[ser]sec gene. Although we were able to generate transfectants overproducing tRNA[ser]sec by as much as tenfold, the additional tRNA was principally retained in the unmethylated form. Selenium supplementation could not significantly affect the relative distributions of the two major selenocysteine tRNA[ser]sec isoacceptors. In addition, increased levels of tRNA[ser]sec did not result in measurable alterations in the levels of selenoproteins, including glutathione peroxidase.

Authors+Show Affiliations

Section on the Molecular Biology of Selenium, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9814763

Citation

Moustafa, M E., et al. "Overproduction of Selenocysteine tRNA in Chinese Hamster Ovary Cells Following Transfection of the Mouse tRNA[Ser]Sec Gene." RNA (New York, N.Y.), vol. 4, no. 11, 1998, pp. 1436-43.
Moustafa ME, El-Saadani MA, Kandeel KM, et al. Overproduction of selenocysteine tRNA in Chinese hamster ovary cells following transfection of the mouse tRNA[Ser]Sec gene. RNA. 1998;4(11):1436-43.
Moustafa, M. E., El-Saadani, M. A., Kandeel, K. M., Mansur, D. B., Lee, B. J., Hatfield, D. L., & Diamond, A. M. (1998). Overproduction of selenocysteine tRNA in Chinese hamster ovary cells following transfection of the mouse tRNA[Ser]Sec gene. RNA (New York, N.Y.), 4(11), 1436-43.
Moustafa ME, et al. Overproduction of Selenocysteine tRNA in Chinese Hamster Ovary Cells Following Transfection of the Mouse tRNA[Ser]Sec Gene. RNA. 1998;4(11):1436-43. PubMed PMID: 9814763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overproduction of selenocysteine tRNA in Chinese hamster ovary cells following transfection of the mouse tRNA[Ser]Sec gene. AU - Moustafa,M E, AU - El-Saadani,M A, AU - Kandeel,K M, AU - Mansur,D B, AU - Lee,B J, AU - Hatfield,D L, AU - Diamond,A M, PY - 1998/11/14/pubmed PY - 1998/11/14/medline PY - 1998/11/14/entrez SP - 1436 EP - 43 JF - RNA (New York, N.Y.) JO - RNA VL - 4 IS - 11 N2 - Selenocysteine insertion during selenoprotein biosynthesis begins with the aminoacylation of selenocysteine tRNA[ser]sec with serine, the conversion of the serine moiety to selenocysteine, and the recognition of specific UGA codons within the mRNA. Selenocysteine tRNA[ser]sec exists as two major forms, differing by methylation of the ribose portion of the nucleotide at the wobble position of the anticodon. The levels and relative distribution of these two forms of the tRNA are influenced by selenium in mammalian cells and tissues. We have generated Chinese hamster ovary cells that exhibit increased levels of tRNA[ser]sec following transfection of the mouse tRNA[ser]sec gene. The levels of selenocysteine tRNA[ser]sec in transfectants increased proportionally to the number of stably integrated copies of the tRNA[ser]sec gene. Although we were able to generate transfectants overproducing tRNA[ser]sec by as much as tenfold, the additional tRNA was principally retained in the unmethylated form. Selenium supplementation could not significantly affect the relative distributions of the two major selenocysteine tRNA[ser]sec isoacceptors. In addition, increased levels of tRNA[ser]sec did not result in measurable alterations in the levels of selenoproteins, including glutathione peroxidase. SN - 1355-8382 UR - https://www.unboundmedicine.com/medline/citation/9814763/Overproduction_of_selenocysteine_tRNA_in_Chinese_hamster_ovary_cells_following_transfection_of_the_mouse_tRNA[Ser]Sec_gene_ L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=9814763 DB - PRIME DP - Unbound Medicine ER -