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Flow cytometric DNA analysis of breast cancers with predominance of carcinoma in situ: a comparison of the premalignant and malignant components.
Clin Cancer Res. 1995 Aug; 1(8):881-8.CC

Abstract

Flow cytometric DNA analysis was performed on unfixed frozen tissue samples from 48 cases of invasive breast cancer (IC) with a predominance of ductal carcinoma in situ (DCIS). In 15 cases the samples contained only the DCIS component, in 17 cases only the IC component, whereas in 16 cases separate samples from the DCIS as well as the IC part within the individual lesion were available. In the latter 16 cases, complete or partial accordance in DNA ploidy between DCIS and IC was found in 12 cases, whereas no correspondence could be demonstrated in the remaining 4 cases, possibly due to intratumoral DNA heterogeneity. Comparison of the DNA index distribution in samples of DCIS and IC from the 48 cases showed concordant results except for the DNA hyperdiploid subclass, in which 6 clones were found in the DCIS portion compared to 18 clones in the IC portion. S-phase fractions were also comparable in the two groups. A comparison of the DCIS component from the present series of breast cancers to our previous series of pure DCIS also showed similar results with respect to the DNA index distribution, DNA heterogeneity, and S-phase fraction. No differences could be demonstrated between DCIS with and without invasion. The results indicate that the DNA ploidy pattern of breast cancer, as detected by flow cytometric DNA analysis, is established at the preinvasive stage of carcinogenesis.

Authors+Show Affiliations

Institute of Pathology, Odense University Hospital, 5000 Odense C, Rigshospitalet, 2100 Copenhagen ø, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9816058

Citation

Ottesen, G L., et al. "Flow Cytometric DNA Analysis of Breast Cancers With Predominance of Carcinoma in Situ: a Comparison of the Premalignant and Malignant Components." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 1, no. 8, 1995, pp. 881-8.
Ottesen GL, Christensen IJ, Larsen JK, et al. Flow cytometric DNA analysis of breast cancers with predominance of carcinoma in situ: a comparison of the premalignant and malignant components. Clin Cancer Res. 1995;1(8):881-8.
Ottesen, G. L., Christensen, I. J., Larsen, J. K., Hansen, B., & Andersen, A. J. (1995). Flow cytometric DNA analysis of breast cancers with predominance of carcinoma in situ: a comparison of the premalignant and malignant components. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 1(8), 881-8.
Ottesen GL, et al. Flow Cytometric DNA Analysis of Breast Cancers With Predominance of Carcinoma in Situ: a Comparison of the Premalignant and Malignant Components. Clin Cancer Res. 1995;1(8):881-8. PubMed PMID: 9816058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric DNA analysis of breast cancers with predominance of carcinoma in situ: a comparison of the premalignant and malignant components. AU - Ottesen,G L, AU - Christensen,I J, AU - Larsen,J K, AU - Hansen,B, AU - Andersen,A J, PY - 1995/8/1/pubmed PY - 1998/11/17/medline PY - 1995/8/1/entrez SP - 881 EP - 8 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin Cancer Res VL - 1 IS - 8 N2 - Flow cytometric DNA analysis was performed on unfixed frozen tissue samples from 48 cases of invasive breast cancer (IC) with a predominance of ductal carcinoma in situ (DCIS). In 15 cases the samples contained only the DCIS component, in 17 cases only the IC component, whereas in 16 cases separate samples from the DCIS as well as the IC part within the individual lesion were available. In the latter 16 cases, complete or partial accordance in DNA ploidy between DCIS and IC was found in 12 cases, whereas no correspondence could be demonstrated in the remaining 4 cases, possibly due to intratumoral DNA heterogeneity. Comparison of the DNA index distribution in samples of DCIS and IC from the 48 cases showed concordant results except for the DNA hyperdiploid subclass, in which 6 clones were found in the DCIS portion compared to 18 clones in the IC portion. S-phase fractions were also comparable in the two groups. A comparison of the DCIS component from the present series of breast cancers to our previous series of pure DCIS also showed similar results with respect to the DNA index distribution, DNA heterogeneity, and S-phase fraction. No differences could be demonstrated between DCIS with and without invasion. The results indicate that the DNA ploidy pattern of breast cancer, as detected by flow cytometric DNA analysis, is established at the preinvasive stage of carcinogenesis. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/9816058/Flow_cytometric_DNA_analysis_of_breast_cancers_with_predominance_of_carcinoma_in_situ:_a_comparison_of_the_premalignant_and_malignant_components_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=9816058 DB - PRIME DP - Unbound Medicine ER -