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Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system.
J Clin Microbiol. 1998 Dec; 36(12):3509-13.JC

Abstract

Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose.

Authors+Show Affiliations

Laboratoire National de Santé, L-1011 Luxembourg, Luxembourg.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9817863

Citation

Bouche, F B., et al. "Evaluation of Hemagglutinin Protein-specific Immunoglobulin M for Diagnosis of Measles By an Enzyme-linked Immunosorbent Assay Based On Recombinant Protein Produced in a High-efficiency Mammalian Expression System." Journal of Clinical Microbiology, vol. 36, no. 12, 1998, pp. 3509-13.
Bouche FB, Brons NH, Houard S, et al. Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system. J Clin Microbiol. 1998;36(12):3509-13.
Bouche, F. B., Brons, N. H., Houard, S., Schneider, F., & Muller, C. P. (1998). Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system. Journal of Clinical Microbiology, 36(12), 3509-13.
Bouche FB, et al. Evaluation of Hemagglutinin Protein-specific Immunoglobulin M for Diagnosis of Measles By an Enzyme-linked Immunosorbent Assay Based On Recombinant Protein Produced in a High-efficiency Mammalian Expression System. J Clin Microbiol. 1998;36(12):3509-13. PubMed PMID: 9817863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system. AU - Bouche,F B, AU - Brons,N H, AU - Houard,S, AU - Schneider,F, AU - Muller,C P, PY - 1998/11/18/pubmed PY - 1998/11/18/medline PY - 1998/11/18/entrez SP - 3509 EP - 13 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 36 IS - 12 N2 - Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/9817863/Evaluation_of_hemagglutinin_protein_specific_immunoglobulin_M_for_diagnosis_of_measles_by_an_enzyme_linked_immunosorbent_assay_based_on_recombinant_protein_produced_in_a_high_efficiency_mammalian_expression_system_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=9817863 DB - PRIME DP - Unbound Medicine ER -