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Separation of inorganic and organic anions by capillary zone electrophoresis with simultaneous indirect UV and conductivity detection.
Electrophoresis. 1998 Oct; 19(14):2459-64.E

Abstract

The analysis of low-molecular-mass anionic compounds using capillary zone electrophoresis (CZE) with simultaneous direct conductivity and indirect UV detection is described. A number of carrier electrolyte compositions has been investigated with respect to the crucial parameters for their compatibility with both detection principles discussed in this paper (UV absorptivity and electrophoretic mobility of the buffer coion). Additionally, parameters including pH and buffer concentration affecting both peak symmetry and signal-to-noise ratio obtained for the analytes of interest had to be optimized. Best results could be achieved with a carrier electrolyte consisting of 4-aminobenzoic acid, bis(2-hydroxyethyl)imino-tris(hydroxymethyl)aminomethane (BIS-Tris) and a pH of 7.0 adjusted with LiOH. This running buffer was used for the analysis of low-molecular-mass anionic compounds in a sample of white wine.

Authors+Show Affiliations

Klampfl CW
Department of Analytical Chemistry, Johannes-Kepler-University Linz, Austria. christian.klampfl@jk.uni-linz.ac.at
Katzmayr MU
No affiliation info available
Buchberger W
No affiliation info available

MeSH

4-Aminobenzoic AcidAnionsBuffersChloridesElectrolytesElectrophoresis, CapillaryHydrogen-Ion ConcentrationLactic AcidLithium CompoundsOsmolar ConcentrationOxalatesShikimic AcidSulfatesTromethamineWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9820968

Citation

Klampfl, C W., et al. "Separation of Inorganic and Organic Anions By Capillary Zone Electrophoresis With Simultaneous Indirect UV and Conductivity Detection." Electrophoresis, vol. 19, no. 14, 1998, pp. 2459-64.
Klampfl CW, Katzmayr MU, Buchberger W. Separation of inorganic and organic anions by capillary zone electrophoresis with simultaneous indirect UV and conductivity detection. Electrophoresis. 1998;19(14):2459-64.
Klampfl, C. W., Katzmayr, M. U., & Buchberger, W. (1998). Separation of inorganic and organic anions by capillary zone electrophoresis with simultaneous indirect UV and conductivity detection. Electrophoresis, 19(14), 2459-64.
Klampfl CW, Katzmayr MU, Buchberger W. Separation of Inorganic and Organic Anions By Capillary Zone Electrophoresis With Simultaneous Indirect UV and Conductivity Detection. Electrophoresis. 1998;19(14):2459-64. PubMed PMID: 9820968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of inorganic and organic anions by capillary zone electrophoresis with simultaneous indirect UV and conductivity detection. AU - Klampfl,C W, AU - Katzmayr,M U, AU - Buchberger,W, PY - 1998/11/20/pubmed PY - 1998/11/20/medline PY - 1998/11/20/entrez SP - 2459 EP - 64 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 14 N2 - The analysis of low-molecular-mass anionic compounds using capillary zone electrophoresis (CZE) with simultaneous direct conductivity and indirect UV detection is described. A number of carrier electrolyte compositions has been investigated with respect to the crucial parameters for their compatibility with both detection principles discussed in this paper (UV absorptivity and electrophoretic mobility of the buffer coion). Additionally, parameters including pH and buffer concentration affecting both peak symmetry and signal-to-noise ratio obtained for the analytes of interest had to be optimized. Best results could be achieved with a carrier electrolyte consisting of 4-aminobenzoic acid, bis(2-hydroxyethyl)imino-tris(hydroxymethyl)aminomethane (BIS-Tris) and a pH of 7.0 adjusted with LiOH. This running buffer was used for the analysis of low-molecular-mass anionic compounds in a sample of white wine. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9820968/Separation_of_inorganic_and_organic_anions_by_capillary_zone_electrophoresis_with_simultaneous_indirect_UV_and_conductivity_detection_ L2 - https://doi.org/10.1002/elps.1150191418 DB - PRIME DP - Unbound Medicine ER -