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Adaptation after small bowel resection is attenuated by sialoadenectomy: the role for endogenous epidermal growth factor.
Surgery. 1998 Nov; 124(5):848-54.S

Abstract

BACKGROUND

Epidermal growth factor (EGF) is likely involved during adaptation after small bowel resection (SBR) because some studies have shown enhanced adaptation by EGF administration. Because the major source of endogenous EGF in mice is the submandibular glands, we sought to determine the effect of submandibular gland excision (SAL) and luminal or systemic EGF replacement on adaptation after SBR.

METHODS

A 50% proximal SBR or Sham-SBR (bowel transection and reanastomosis) was performed on male C57BL/6 mice after either SAL or gland mobilization only. Additional mice underwent both SBR and SAL and then received daily EGF or saline solution by intraperitoneal or orogastric administration. At 1 week, adaptation was characterized in the ileum as changes in villus height, DNA, and protein content.

RESULTS

SAL significantly attenuated the increase in ileal villus height, total protein, and DNA content after SBR. Both systemic and oral EGF reversed these findings equally and significantly augmented all parameters of intestinal adaptation after SAL.

CONCLUSIONS

Submandibular EGF is important for the adaptive response to massive SBR. As both luminal and systemic EGF equally reversed the findings following SAL and SBR, the specific site of action for endogenous EGF during adaptation is either the luminal or basolateral surface of the enterocyte.

Authors+Show Affiliations

Children's Hospital Medical Center, Department of Surgery, University of Cincinnati College of Medicine, Ohio 45229-3039, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9823398

Citation

Helmrath, M A., et al. "Adaptation After Small Bowel Resection Is Attenuated By Sialoadenectomy: the Role for Endogenous Epidermal Growth Factor." Surgery, vol. 124, no. 5, 1998, pp. 848-54.
Helmrath MA, Shin CE, Fox JW, et al. Adaptation after small bowel resection is attenuated by sialoadenectomy: the role for endogenous epidermal growth factor. Surgery. 1998;124(5):848-54.
Helmrath, M. A., Shin, C. E., Fox, J. W., Erwin, C. R., & Warner, B. W. (1998). Adaptation after small bowel resection is attenuated by sialoadenectomy: the role for endogenous epidermal growth factor. Surgery, 124(5), 848-54.
Helmrath MA, et al. Adaptation After Small Bowel Resection Is Attenuated By Sialoadenectomy: the Role for Endogenous Epidermal Growth Factor. Surgery. 1998;124(5):848-54. PubMed PMID: 9823398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adaptation after small bowel resection is attenuated by sialoadenectomy: the role for endogenous epidermal growth factor. AU - Helmrath,M A, AU - Shin,C E, AU - Fox,J W, AU - Erwin,C R, AU - Warner,B W, PY - 1998/11/21/pubmed PY - 1998/11/21/medline PY - 1998/11/21/entrez SP - 848 EP - 54 JF - Surgery JO - Surgery VL - 124 IS - 5 N2 - BACKGROUND: Epidermal growth factor (EGF) is likely involved during adaptation after small bowel resection (SBR) because some studies have shown enhanced adaptation by EGF administration. Because the major source of endogenous EGF in mice is the submandibular glands, we sought to determine the effect of submandibular gland excision (SAL) and luminal or systemic EGF replacement on adaptation after SBR. METHODS: A 50% proximal SBR or Sham-SBR (bowel transection and reanastomosis) was performed on male C57BL/6 mice after either SAL or gland mobilization only. Additional mice underwent both SBR and SAL and then received daily EGF or saline solution by intraperitoneal or orogastric administration. At 1 week, adaptation was characterized in the ileum as changes in villus height, DNA, and protein content. RESULTS: SAL significantly attenuated the increase in ileal villus height, total protein, and DNA content after SBR. Both systemic and oral EGF reversed these findings equally and significantly augmented all parameters of intestinal adaptation after SAL. CONCLUSIONS: Submandibular EGF is important for the adaptive response to massive SBR. As both luminal and systemic EGF equally reversed the findings following SAL and SBR, the specific site of action for endogenous EGF during adaptation is either the luminal or basolateral surface of the enterocyte. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/9823398/Adaptation_after_small_bowel_resection_is_attenuated_by_sialoadenectomy:_the_role_for_endogenous_epidermal_growth_factor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(98)70008-X DB - PRIME DP - Unbound Medicine ER -