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Daily variations of serum diamine oxidase and the influence of H1 and H2 blockers: a critical approach to routine diamine oxidase assessment.
Inflamm Res. 1998 Oct; 47(10):396-400.IR

Abstract

OBJECTIVE AND DESIGN

Histamine in food has been shown to induce intolerance reactions mimicking food allergy. These reactions seem to be due to impaired histamine metabolism caused by reduced diamine oxidase activity. To validate routine serum diamine oxidase assessment, daily variations of diamine oxidase were evaluated.

METHODS

Blood was drawn from each of 20 healthy volunteers (10 female, 10 male; mean age 32.5 years) every 2 h from 9 a.m. to 5 p.m., and diamine oxidase activity was measured using the C14 putrescine method. To assess possible influences of H1 and H2 blockers on diamine oxidase activity, diphenhydramine, ketotifen, cimetidine, and ranitidine were incubated at pharmacologic concentrations with human placental diamine oxidase (identical to neutrophilic and eosinophilic diamine oxidase). Inhibition of diamine oxidase activity was calculated as the percentage of inhibition versus control. In addition, the known diamine oxidase inhibitors, dihydralazine and aminoguanidine, were used as positive controls.

RESULTS

Serum diamine oxidase levels showed no significant daily variations (0.041 +/- 0.025; 0.037 +/- 0.022; 0.041 +/- 0.023; 0.040 +/- 0.023; 0.038 +/- 0.025 nKat/l) and no significant sex differences (female 0.040 +/- 0.028 nKat/l versus male 0.039 +/- 0.019 nKat/l). Antihistamines had no influence on diamine oxidase activity except for cimetidine, which caused 25% inhibition at the highest dose tested (p < 0.0002) (positive control: aminoguanidine 85% inhibition (p< 0.0001), dihydralazine 68% inhibition (p<0.0001)) and diphenhydramine, which caused 19% increase (p<0.0001) of enzyme activity.

CONCLUSION

Serum diamine oxidase levels do not show daily variations allowing assessment anytime during office hours. However, diagnostic interpretation of serum diamine oxidase levels may be difficult.

Authors+Show Affiliations

Johns Hopkins University, School of Medicine, Johns Hopkins Asthma & Allergy Center, Division of Clinical Immunology, Baltimore, MD 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9831324

Citation

Wantke, F, et al. "Daily Variations of Serum Diamine Oxidase and the Influence of H1 and H2 Blockers: a Critical Approach to Routine Diamine Oxidase Assessment." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 47, no. 10, 1998, pp. 396-400.
Wantke F, Proud D, Siekierski E, et al. Daily variations of serum diamine oxidase and the influence of H1 and H2 blockers: a critical approach to routine diamine oxidase assessment. Inflamm Res. 1998;47(10):396-400.
Wantke, F., Proud, D., Siekierski, E., & Kagey-Sobotka, A. (1998). Daily variations of serum diamine oxidase and the influence of H1 and H2 blockers: a critical approach to routine diamine oxidase assessment. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 47(10), 396-400.
Wantke F, et al. Daily Variations of Serum Diamine Oxidase and the Influence of H1 and H2 Blockers: a Critical Approach to Routine Diamine Oxidase Assessment. Inflamm Res. 1998;47(10):396-400. PubMed PMID: 9831324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Daily variations of serum diamine oxidase and the influence of H1 and H2 blockers: a critical approach to routine diamine oxidase assessment. AU - Wantke,F, AU - Proud,D, AU - Siekierski,E, AU - Kagey-Sobotka,A, PY - 1998/11/27/pubmed PY - 1998/11/27/medline PY - 1998/11/27/entrez SP - 396 EP - 400 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 47 IS - 10 N2 - OBJECTIVE AND DESIGN: Histamine in food has been shown to induce intolerance reactions mimicking food allergy. These reactions seem to be due to impaired histamine metabolism caused by reduced diamine oxidase activity. To validate routine serum diamine oxidase assessment, daily variations of diamine oxidase were evaluated. METHODS: Blood was drawn from each of 20 healthy volunteers (10 female, 10 male; mean age 32.5 years) every 2 h from 9 a.m. to 5 p.m., and diamine oxidase activity was measured using the C14 putrescine method. To assess possible influences of H1 and H2 blockers on diamine oxidase activity, diphenhydramine, ketotifen, cimetidine, and ranitidine were incubated at pharmacologic concentrations with human placental diamine oxidase (identical to neutrophilic and eosinophilic diamine oxidase). Inhibition of diamine oxidase activity was calculated as the percentage of inhibition versus control. In addition, the known diamine oxidase inhibitors, dihydralazine and aminoguanidine, were used as positive controls. RESULTS: Serum diamine oxidase levels showed no significant daily variations (0.041 +/- 0.025; 0.037 +/- 0.022; 0.041 +/- 0.023; 0.040 +/- 0.023; 0.038 +/- 0.025 nKat/l) and no significant sex differences (female 0.040 +/- 0.028 nKat/l versus male 0.039 +/- 0.019 nKat/l). Antihistamines had no influence on diamine oxidase activity except for cimetidine, which caused 25% inhibition at the highest dose tested (p < 0.0002) (positive control: aminoguanidine 85% inhibition (p< 0.0001), dihydralazine 68% inhibition (p<0.0001)) and diphenhydramine, which caused 19% increase (p<0.0001) of enzyme activity. CONCLUSION: Serum diamine oxidase levels do not show daily variations allowing assessment anytime during office hours. However, diagnostic interpretation of serum diamine oxidase levels may be difficult. SN - 1023-3830 UR - https://www.unboundmedicine.com/medline/citation/9831324/Daily_variations_of_serum_diamine_oxidase_and_the_influence_of_H1_and_H2_blockers:_a_critical_approach_to_routine_diamine_oxidase_assessment_ L2 - https://dx.doi.org/10.1007/s000110050350 DB - PRIME DP - Unbound Medicine ER -