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Involvement of the IP-10 chemokine in sarcoid granulomatous reactions.
J Immunol. 1998 Dec 01; 161(11):6413-20.JI

Abstract

The accumulation of T cells and monocytes at sites of ongoing inflammation represents the earliest step in the series of events that lead to granuloma formation in sarcoidosis. In this study, we evaluated the pulmonary production of IFN-inducible protein 10 (IP-10), a CXC chemokine that stimulates the directional migration of activated T cells. Striking levels of IP-10 were demonstrated in the bronchoalveolar lavage (BAL) fluid of 24 patients with pulmonary sarcoidosis and lymphocytic alveolitis, as compared with patients with inactive disease or control subjects. A positive correlation was demonstrated between IP-10 levels and the number of sarcoid CD45R0+/CD4+ cells in the BAL. Immunochemistry, performed with an anti-human IP-10 polyclonal Ab in lymph nodes displaying prominent sarcoid granulomas, showed that cells bearing IP-10 were mainly epithelioid cells and CD68+ macrophages located inside granulomatous areas. Macrophages recovered from the BAL of sarcoid patients stained positive for IP-10 protein. Furthermore, alveolar macrophages isolated from sarcoid patients with T cell alveolitis and cultured for 24 h in presence of IFN-gamma secreted definite levels of IP-10 capable of inducing T cell chemiotaxis. Interestingly, alveolar lymphocytes recovered from patients with active sarcoidosis were CD4+ T cells expressing Th1 cytokines (IL-2 and IFN-gamma) and high levels of CXCR3. Taken together, these data suggest the potential role of IP-10 in regulating the migration and activation of T cells toward sites of sarcoid inflammatory process and the consequent granuloma formation.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine, Padova University School of Medicine, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9834133

Citation

Agostini, C, et al. "Involvement of the IP-10 Chemokine in Sarcoid Granulomatous Reactions." Journal of Immunology (Baltimore, Md. : 1950), vol. 161, no. 11, 1998, pp. 6413-20.
Agostini C, Cassatella M, Zambello R, et al. Involvement of the IP-10 chemokine in sarcoid granulomatous reactions. J Immunol. 1998;161(11):6413-20.
Agostini, C., Cassatella, M., Zambello, R., Trentin, L., Gasperini, S., Perin, A., Piazza, F., Siviero, M., Facco, M., Dziejman, M., Chilosi, M., Qin, S., Luster, A. D., & Semenzato, G. (1998). Involvement of the IP-10 chemokine in sarcoid granulomatous reactions. Journal of Immunology (Baltimore, Md. : 1950), 161(11), 6413-20.
Agostini C, et al. Involvement of the IP-10 Chemokine in Sarcoid Granulomatous Reactions. J Immunol. 1998 Dec 1;161(11):6413-20. PubMed PMID: 9834133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of the IP-10 chemokine in sarcoid granulomatous reactions. AU - Agostini,C, AU - Cassatella,M, AU - Zambello,R, AU - Trentin,L, AU - Gasperini,S, AU - Perin,A, AU - Piazza,F, AU - Siviero,M, AU - Facco,M, AU - Dziejman,M, AU - Chilosi,M, AU - Qin,S, AU - Luster,A D, AU - Semenzato,G, PY - 1998/12/2/pubmed PY - 1998/12/2/medline PY - 1998/12/2/entrez SP - 6413 EP - 20 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 161 IS - 11 N2 - The accumulation of T cells and monocytes at sites of ongoing inflammation represents the earliest step in the series of events that lead to granuloma formation in sarcoidosis. In this study, we evaluated the pulmonary production of IFN-inducible protein 10 (IP-10), a CXC chemokine that stimulates the directional migration of activated T cells. Striking levels of IP-10 were demonstrated in the bronchoalveolar lavage (BAL) fluid of 24 patients with pulmonary sarcoidosis and lymphocytic alveolitis, as compared with patients with inactive disease or control subjects. A positive correlation was demonstrated between IP-10 levels and the number of sarcoid CD45R0+/CD4+ cells in the BAL. Immunochemistry, performed with an anti-human IP-10 polyclonal Ab in lymph nodes displaying prominent sarcoid granulomas, showed that cells bearing IP-10 were mainly epithelioid cells and CD68+ macrophages located inside granulomatous areas. Macrophages recovered from the BAL of sarcoid patients stained positive for IP-10 protein. Furthermore, alveolar macrophages isolated from sarcoid patients with T cell alveolitis and cultured for 24 h in presence of IFN-gamma secreted definite levels of IP-10 capable of inducing T cell chemiotaxis. Interestingly, alveolar lymphocytes recovered from patients with active sarcoidosis were CD4+ T cells expressing Th1 cytokines (IL-2 and IFN-gamma) and high levels of CXCR3. Taken together, these data suggest the potential role of IP-10 in regulating the migration and activation of T cells toward sites of sarcoid inflammatory process and the consequent granuloma formation. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9834133/Involvement_of_the_IP_10_chemokine_in_sarcoid_granulomatous_reactions_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9834133 DB - PRIME DP - Unbound Medicine ER -