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Autoantibodies to tissue transglutaminase as predictors of celiac disease.
Gastroenterology. 1998 Dec; 115(6):1317-21.G

Abstract

BACKGROUND & AIMS

Immunoglobulin A (IgA) autoantibodies to endomysium (EMA) are highly specific and sensitive markers for celiac disease. Recently, we identified tissue transglutaminase (tTG) as the major if not sole endomysial autoantigen.

METHODS

An enzyme-linked immunosorbent assay (ELISA) was established to measure IgA anti-tTG titers in serum samples from 106 celiac patients with partial or subtotal villous atrophy, 43 celiac patients on a gluten-free diet, and 114 diseased and healthy controls. Results were correlated with clinical and histological data and with EMA titers.

RESULTS

In patients with biopsy-proven celiac disease consuming a normal, gluten-containing diet, 98.1% of the serum samples had elevated IgA titers against tTG, whereas 94.7% of the control sera were negative. IgA anti-tTG correlated positively with semiquantitative IgA EMA titers (r = 0.862; P < 0.0001).

CONCLUSIONS

An ELISA based on tTG allows diagnosis of celiac disease with a high sensitivity and specificity. IgA anti-tTG and IgA EMA show an excellent correlation, further confirming the enzyme as the celiac disease autoantigen. Because the assay is quantitative, not subjected to interobserver variation, and easy to perform, it will be a useful tool for population screening of a hitherto underdiagnosed disease.

Authors+Show Affiliations

Department of Gastroenterology, Klinikum Benjamin Franklin, Free University of Berlin, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9834256

Citation

Dieterich, W, et al. "Autoantibodies to Tissue Transglutaminase as Predictors of Celiac Disease." Gastroenterology, vol. 115, no. 6, 1998, pp. 1317-21.
Dieterich W, Laag E, Schöpper H, et al. Autoantibodies to tissue transglutaminase as predictors of celiac disease. Gastroenterology. 1998;115(6):1317-21.
Dieterich, W., Laag, E., Schöpper, H., Volta, U., Ferguson, A., Gillett, H., Riecken, E. O., & Schuppan, D. (1998). Autoantibodies to tissue transglutaminase as predictors of celiac disease. Gastroenterology, 115(6), 1317-21.
Dieterich W, et al. Autoantibodies to Tissue Transglutaminase as Predictors of Celiac Disease. Gastroenterology. 1998;115(6):1317-21. PubMed PMID: 9834256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoantibodies to tissue transglutaminase as predictors of celiac disease. AU - Dieterich,W, AU - Laag,E, AU - Schöpper,H, AU - Volta,U, AU - Ferguson,A, AU - Gillett,H, AU - Riecken,E O, AU - Schuppan,D, PY - 1998/12/3/pubmed PY - 1998/12/3/medline PY - 1998/12/3/entrez SP - 1317 EP - 21 JF - Gastroenterology JO - Gastroenterology VL - 115 IS - 6 N2 - BACKGROUND & AIMS: Immunoglobulin A (IgA) autoantibodies to endomysium (EMA) are highly specific and sensitive markers for celiac disease. Recently, we identified tissue transglutaminase (tTG) as the major if not sole endomysial autoantigen. METHODS: An enzyme-linked immunosorbent assay (ELISA) was established to measure IgA anti-tTG titers in serum samples from 106 celiac patients with partial or subtotal villous atrophy, 43 celiac patients on a gluten-free diet, and 114 diseased and healthy controls. Results were correlated with clinical and histological data and with EMA titers. RESULTS: In patients with biopsy-proven celiac disease consuming a normal, gluten-containing diet, 98.1% of the serum samples had elevated IgA titers against tTG, whereas 94.7% of the control sera were negative. IgA anti-tTG correlated positively with semiquantitative IgA EMA titers (r = 0.862; P < 0.0001). CONCLUSIONS: An ELISA based on tTG allows diagnosis of celiac disease with a high sensitivity and specificity. IgA anti-tTG and IgA EMA show an excellent correlation, further confirming the enzyme as the celiac disease autoantigen. Because the assay is quantitative, not subjected to interobserver variation, and easy to perform, it will be a useful tool for population screening of a hitherto underdiagnosed disease. SN - 0016-5085 UR - https://www.unboundmedicine.com/medline/citation/9834256/Autoantibodies_to_tissue_transglutaminase_as_predictors_of_celiac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016508598005630 DB - PRIME DP - Unbound Medicine ER -