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Decrease of telomere length in thyroid adenomas without telomerase activity.
J Clin Endocrinol Metab. 1998 Dec; 83(12):4368-72.JC

Abstract

In somatic cells, telomeres shorten with population doubling, thus limiting their capacity to divide. Telomerase, which synthesizes telomeric repeats, can compensate for such shortening. Telomerase activity is known to be absent from most somatic differentiated cells but is present in germline cells, immortal cell lines, or a large majority of malignant tumors. Autonomous thyroid adenomas are benign tumors composed of highly differentiated cells characterized by TSH-independent function and growth. Telomere length and telomerase activity were measured in autonomous and hypofunctioning adenomas and their surrounding tissues. A significant decrease of 3.8+/-1.0 kilobases (kb) was observed in the length of the terminal restriction fragments (TRF) in 12 autonomous adenomas (8.6+/-1.1 kb), compared with the TRF length of their surrounding tissues (12.4+/-1.6 kb). The same kind of decrease, 3.5+/-1.2 kb, was also observed in 16 hypofunctioning adenomas (12.3+/-1.7 kb in surrounding tissue and 8.8+/-1.6 kb in the adenomas). No telomerase activity was detected either in the 12 autonomous adenomas studied or in most of the quiescent tissues (10 of 12). Most of the hypofunctioning adenomas tested (15 of 16) did not display telomerase activity. These results suggest that the cells have undergone a higher number of cell divisions in the adenomas than in the surrounding tissue. Moreover, there is a larger spread of the TRF length distribution in autonomous adenomas than in the collateral tissue. This could reflect the heterogeneity in proliferation status of the cells in the nodule, some of which have reached the end of their life span, whereas others are still proliferating (but with no malignant potential for the autonomous adenomas). In conclusion, benign adenomas exhibit a shorter and more variable telomere length than the normal collateral quiescent tissue, with no telomerase activity to compensate this loss in telomere length.

Authors+Show Affiliations

Institut de Recherche Interdisciplinaire, Université Libre de Bruxelles, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9851779

Citation

De Deken, X, et al. "Decrease of Telomere Length in Thyroid Adenomas Without Telomerase Activity." The Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 12, 1998, pp. 4368-72.
De Deken X, Vilain C, Van Sande J, et al. Decrease of telomere length in thyroid adenomas without telomerase activity. J Clin Endocrinol Metab. 1998;83(12):4368-72.
De Deken, X., Vilain, C., Van Sande, J., Dumont, J. E., & Miot, F. (1998). Decrease of telomere length in thyroid adenomas without telomerase activity. The Journal of Clinical Endocrinology and Metabolism, 83(12), 4368-72.
De Deken X, et al. Decrease of Telomere Length in Thyroid Adenomas Without Telomerase Activity. J Clin Endocrinol Metab. 1998;83(12):4368-72. PubMed PMID: 9851779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decrease of telomere length in thyroid adenomas without telomerase activity. AU - De Deken,X, AU - Vilain,C, AU - Van Sande,J, AU - Dumont,J E, AU - Miot,F, PY - 1998/12/16/pubmed PY - 1998/12/16/medline PY - 1998/12/16/entrez SP - 4368 EP - 72 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 83 IS - 12 N2 - In somatic cells, telomeres shorten with population doubling, thus limiting their capacity to divide. Telomerase, which synthesizes telomeric repeats, can compensate for such shortening. Telomerase activity is known to be absent from most somatic differentiated cells but is present in germline cells, immortal cell lines, or a large majority of malignant tumors. Autonomous thyroid adenomas are benign tumors composed of highly differentiated cells characterized by TSH-independent function and growth. Telomere length and telomerase activity were measured in autonomous and hypofunctioning adenomas and their surrounding tissues. A significant decrease of 3.8+/-1.0 kilobases (kb) was observed in the length of the terminal restriction fragments (TRF) in 12 autonomous adenomas (8.6+/-1.1 kb), compared with the TRF length of their surrounding tissues (12.4+/-1.6 kb). The same kind of decrease, 3.5+/-1.2 kb, was also observed in 16 hypofunctioning adenomas (12.3+/-1.7 kb in surrounding tissue and 8.8+/-1.6 kb in the adenomas). No telomerase activity was detected either in the 12 autonomous adenomas studied or in most of the quiescent tissues (10 of 12). Most of the hypofunctioning adenomas tested (15 of 16) did not display telomerase activity. These results suggest that the cells have undergone a higher number of cell divisions in the adenomas than in the surrounding tissue. Moreover, there is a larger spread of the TRF length distribution in autonomous adenomas than in the collateral tissue. This could reflect the heterogeneity in proliferation status of the cells in the nodule, some of which have reached the end of their life span, whereas others are still proliferating (but with no malignant potential for the autonomous adenomas). In conclusion, benign adenomas exhibit a shorter and more variable telomere length than the normal collateral quiescent tissue, with no telomerase activity to compensate this loss in telomere length. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/9851779/Decrease_of_telomere_length_in_thyroid_adenomas_without_telomerase_activity_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.83.12.5349 DB - PRIME DP - Unbound Medicine ER -