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Ultrastructure of Calymmatobacterium granulomatis: comparison of culture with tissue biopsy specimens.
J Med Microbiol. 1998 Dec; 47(12):1069-73.JM

Abstract

The ultrastructural features of cells of Calymmatobacterium granulomatis from monocyte co-cultures and tissue biopsy specimens were compared. In cultures the bacteria were mainly extracellular, i.e., not within membrane-bound vacuoles. The bacterial body was surrounded by a uniformly extensive homogeneous layer with a relatively high electron density. This layer varied considerably in tissue biopsy specimens, having either homogeneously electron-dense or delicate web-like structures with varying density and thickness. In tissue specimens the bacteria were located predominantly within vacuoles of varying sizes in the cytoplasm of the macrophages and, occasionally, extracellularly within the intercellular spaces of the stroma. The bacterial cytoplasm contained ribosomes scattered throughout with electron-dense granules located peripherally. The trilaminar cell-wall structure was typical of a gram-negative organism, comprising an outer membrane, a middle electron-opaque layer and an inner plasma membrane. Surface structures such as fimbriae, flagella and bacteriophages were not identified in specimens from either source.

Authors+Show Affiliations

Department of Medical Microbiology and Optics and Imaging Centre, Faculty of Medicine, University of Natal, Durban, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9856642

Citation

Kharsany, A B., et al. "Ultrastructure of Calymmatobacterium Granulomatis: Comparison of Culture With Tissue Biopsy Specimens." Journal of Medical Microbiology, vol. 47, no. 12, 1998, pp. 1069-73.
Kharsany AB, Hoosen AA, Naicker T, et al. Ultrastructure of Calymmatobacterium granulomatis: comparison of culture with tissue biopsy specimens. J Med Microbiol. 1998;47(12):1069-73.
Kharsany, A. B., Hoosen, A. A., Naicker, T., Kiepiela, P., & Sturm, A. W. (1998). Ultrastructure of Calymmatobacterium granulomatis: comparison of culture with tissue biopsy specimens. Journal of Medical Microbiology, 47(12), 1069-73.
Kharsany AB, et al. Ultrastructure of Calymmatobacterium Granulomatis: Comparison of Culture With Tissue Biopsy Specimens. J Med Microbiol. 1998;47(12):1069-73. PubMed PMID: 9856642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructure of Calymmatobacterium granulomatis: comparison of culture with tissue biopsy specimens. AU - Kharsany,A B, AU - Hoosen,A A, AU - Naicker,T, AU - Kiepiela,P, AU - Sturm,A W, PY - 1998/12/18/pubmed PY - 1998/12/18/medline PY - 1998/12/18/entrez SP - 1069 EP - 73 JF - Journal of medical microbiology JO - J Med Microbiol VL - 47 IS - 12 N2 - The ultrastructural features of cells of Calymmatobacterium granulomatis from monocyte co-cultures and tissue biopsy specimens were compared. In cultures the bacteria were mainly extracellular, i.e., not within membrane-bound vacuoles. The bacterial body was surrounded by a uniformly extensive homogeneous layer with a relatively high electron density. This layer varied considerably in tissue biopsy specimens, having either homogeneously electron-dense or delicate web-like structures with varying density and thickness. In tissue specimens the bacteria were located predominantly within vacuoles of varying sizes in the cytoplasm of the macrophages and, occasionally, extracellularly within the intercellular spaces of the stroma. The bacterial cytoplasm contained ribosomes scattered throughout with electron-dense granules located peripherally. The trilaminar cell-wall structure was typical of a gram-negative organism, comprising an outer membrane, a middle electron-opaque layer and an inner plasma membrane. Surface structures such as fimbriae, flagella and bacteriophages were not identified in specimens from either source. SN - 0022-2615 UR - https://www.unboundmedicine.com/medline/citation/9856642/Ultrastructure_of_Calymmatobacterium_granulomatis:_comparison_of_culture_with_tissue_biopsy_specimens. L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/00222615-47-12-1069 DB - PRIME DP - Unbound Medicine ER -