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In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes.
Anticancer Res. 1998 Sep-Oct; 18(5A):3171-80.AR

Abstract

AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of T cells, we have used in situ reverse transcription based polymerase chain reaction (RT-PCR) to study the initial EBV transcriptional program in homogeneous CD4+ and CD8+ lymphocytes. Following EBV infection, Epstein-Barr nuclear antigen (EBNA) expression could be detected in T rosetting CD4+ and CD8+ T lymphocytes. Only a few cells showed viral capsid antigen (VCA). EBV immediate-early gene transcripts (BZLF1, BRLF1, and BMLF1) encoded in the BamHI Z, R, and M fragments could be detected by in situ RT-PCR in the EBV producer cell line B95.8. Both BZLF1 and BRLF1 immediate-early transcripts, but not BMLF1 transcript, could be detected in individual CD4+ and CD8+ T cells infected with EBV. Demonstration of EBV mRNA transcripts encoding immediate-early transcriptional transactivators in EBV-infected T cells provides the first evidence for a possible mechanism whereby EBV could contribute to T cell proliferation and EBV-associated T cell malignancies.

Authors+Show Affiliations

Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9858880

Citation

Guan, M, et al. "In Situ RT-PCR Detection of Epstein-Barr Virus Immediate-early Transcripts in CD4+ and CD8+ T Lymphocytes." Anticancer Research, vol. 18, no. 5A, 1998, pp. 3171-80.
Guan M, Romano G, Henderson EE. In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes. Anticancer Res. 1998;18(5A):3171-80.
Guan, M., Romano, G., & Henderson, E. E. (1998). In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes. Anticancer Research, 18(5A), 3171-80.
Guan M, Romano G, Henderson EE. In Situ RT-PCR Detection of Epstein-Barr Virus Immediate-early Transcripts in CD4+ and CD8+ T Lymphocytes. Anticancer Res. 1998 Sep-Oct;18(5A):3171-80. PubMed PMID: 9858880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes. AU - Guan,M, AU - Romano,G, AU - Henderson,E E, PY - 1998/12/22/pubmed PY - 1998/12/22/medline PY - 1998/12/22/entrez SP - 3171 EP - 80 JF - Anticancer research JO - Anticancer Res VL - 18 IS - 5A N2 - AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of T cells, we have used in situ reverse transcription based polymerase chain reaction (RT-PCR) to study the initial EBV transcriptional program in homogeneous CD4+ and CD8+ lymphocytes. Following EBV infection, Epstein-Barr nuclear antigen (EBNA) expression could be detected in T rosetting CD4+ and CD8+ T lymphocytes. Only a few cells showed viral capsid antigen (VCA). EBV immediate-early gene transcripts (BZLF1, BRLF1, and BMLF1) encoded in the BamHI Z, R, and M fragments could be detected by in situ RT-PCR in the EBV producer cell line B95.8. Both BZLF1 and BRLF1 immediate-early transcripts, but not BMLF1 transcript, could be detected in individual CD4+ and CD8+ T cells infected with EBV. Demonstration of EBV mRNA transcripts encoding immediate-early transcriptional transactivators in EBV-infected T cells provides the first evidence for a possible mechanism whereby EBV could contribute to T cell proliferation and EBV-associated T cell malignancies. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/9858880/In_situ_RT_PCR_detection_of_Epstein_Barr_virus_immediate_early_transcripts_in_CD4+_and_CD8+_T_lymphocytes_ L2 - https://antibodies.cancer.gov/detail/CPTC-CD4-1 DB - PRIME DP - Unbound Medicine ER -