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HLA restriction patterns of gliadin- and astrovirus-specific CD4+ T cells isolated in parallel from the small intestine of celiac disease patients.
Tissue Antigens. 1998 Nov; 52(5):407-15.TA

Abstract

Celiac disease is a common HLA-DQ2-associated enteropathy caused by an abnormal T-cell-mediated immune response to ingested wheat gliadin proteins. We have previously isolated in situ activated mucosal T cells from celiac disease patients and demonstrated that these T cells were gliadin specific and predominantly DQ2 restricted. In contrast to this, gliadin-specific T cells isolated from peripheral blood display a variable HLA restriction pattern, thereby indicating that the skewed DQ restriction of T cells resident in the celiac lesions could be dictated by a preference for DQ-mediated antigen presentation in the mucosa of CD patients. To address this, we analyzed the HLA restriction of T cells recognizing astrovirus, a common gastroentetitis virus, isolated from intestinal mucosa of six celiac disease patients. As an internal control, gliadin-specific T cells were isolated and analyzed in parallel. The gliadin-specific mucosal T cells were marked in their DQ2 restriction, whereas the parallel astrovirus-specific T cells were predominantly restricted by DR molecules. Our data indicate that the repertoire of T cells present in celiac lesions is determined by the priming antigen(s) and not by a skewing in the expression of functional HLA class II isotypes in the disease affected small intestinal mucosa.

Authors+Show Affiliations

Institute of Transplantation Immunology, The National Hospital, University of Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9864029

Citation

Molberg, O, et al. "HLA Restriction Patterns of Gliadin- and Astrovirus-specific CD4+ T Cells Isolated in Parallel From the Small Intestine of Celiac Disease Patients." Tissue Antigens, vol. 52, no. 5, 1998, pp. 407-15.
Molberg O, Lundin KE, Nilsen EM, et al. HLA restriction patterns of gliadin- and astrovirus-specific CD4+ T cells isolated in parallel from the small intestine of celiac disease patients. Tissue Antigens. 1998;52(5):407-15.
Molberg, O., Lundin, K. E., Nilsen, E. M., Scott, H., Kett, K., Brandtzaeg, P., Thorsby, E., & Sollid, L. M. (1998). HLA restriction patterns of gliadin- and astrovirus-specific CD4+ T cells isolated in parallel from the small intestine of celiac disease patients. Tissue Antigens, 52(5), 407-15.
Molberg O, et al. HLA Restriction Patterns of Gliadin- and Astrovirus-specific CD4+ T Cells Isolated in Parallel From the Small Intestine of Celiac Disease Patients. Tissue Antigens. 1998;52(5):407-15. PubMed PMID: 9864029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA restriction patterns of gliadin- and astrovirus-specific CD4+ T cells isolated in parallel from the small intestine of celiac disease patients. AU - Molberg,O, AU - Lundin,K E, AU - Nilsen,E M, AU - Scott,H, AU - Kett,K, AU - Brandtzaeg,P, AU - Thorsby,E, AU - Sollid,L M, PY - 1998/12/24/pubmed PY - 1998/12/24/medline PY - 1998/12/24/entrez SP - 407 EP - 15 JF - Tissue antigens JO - Tissue Antigens VL - 52 IS - 5 N2 - Celiac disease is a common HLA-DQ2-associated enteropathy caused by an abnormal T-cell-mediated immune response to ingested wheat gliadin proteins. We have previously isolated in situ activated mucosal T cells from celiac disease patients and demonstrated that these T cells were gliadin specific and predominantly DQ2 restricted. In contrast to this, gliadin-specific T cells isolated from peripheral blood display a variable HLA restriction pattern, thereby indicating that the skewed DQ restriction of T cells resident in the celiac lesions could be dictated by a preference for DQ-mediated antigen presentation in the mucosa of CD patients. To address this, we analyzed the HLA restriction of T cells recognizing astrovirus, a common gastroentetitis virus, isolated from intestinal mucosa of six celiac disease patients. As an internal control, gliadin-specific T cells were isolated and analyzed in parallel. The gliadin-specific mucosal T cells were marked in their DQ2 restriction, whereas the parallel astrovirus-specific T cells were predominantly restricted by DR molecules. Our data indicate that the repertoire of T cells present in celiac lesions is determined by the priming antigen(s) and not by a skewing in the expression of functional HLA class II isotypes in the disease affected small intestinal mucosa. SN - 0001-2815 UR - https://www.unboundmedicine.com/medline/citation/9864029/HLA_restriction_patterns_of_gliadin__and_astrovirus_specific_CD4+_T_cells_isolated_in_parallel_from_the_small_intestine_of_celiac_disease_patients_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0001-2815&date=1998&volume=52&issue=5&spage=407 DB - PRIME DP - Unbound Medicine ER -