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Analysis of cefadroxil by micellar electrokinetic capillary chromatography: development and validation.
Electrophoresis. 1998 Nov; 19(16-17):2890-4.E

Abstract

A method is developed and validated for analysis of the antibiotic cefadroxil using micellar electrokinetic capillary chromatography. It permits cefadroxil to be completely separated from ten of its known related substances within 15 min (including the washing procedure). The separation is performed in an acetate buffer (50 mM, pH 5.25) containing sodium dodecyl sulfate (SDS; 110 mM). The fused-silica capillary was 44 cm long (36 cm effective length), 50 microm ID; the voltage, 18 kV; temperature, 15 degrees C; and the detection wavelength; 254 nm. The influence of the type of buffer, buffer pH and concentration, and of the SDS concentration was investigated. The robustness of the method was examined by means of a full-fraction factorial design. The parameters for validation such as linearity, precision, limit of detection and limit of quantitation are also reported.

Authors+Show Affiliations

Li YM
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, K.U. Leuven, Belgium.
Vanderghinste D
No affiliation info available
Pecanac D
No affiliation info available
Van Schepdael A
No affiliation info available
Roets E
No affiliation info available
Hoogmartens J
No affiliation info available

MeSH

CefadroxilCephalosporinsChromatography, Micellar Electrokinetic CapillaryElectrophoresis, CapillaryReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9870384

Citation

Li, Y M., et al. "Analysis of Cefadroxil By Micellar Electrokinetic Capillary Chromatography: Development and Validation." Electrophoresis, vol. 19, no. 16-17, 1998, pp. 2890-4.
Li YM, Vanderghinste D, Pecanac D, et al. Analysis of cefadroxil by micellar electrokinetic capillary chromatography: development and validation. Electrophoresis. 1998;19(16-17):2890-4.
Li, Y. M., Vanderghinste, D., Pecanac, D., Van Schepdael, A., Roets, E., & Hoogmartens, J. (1998). Analysis of cefadroxil by micellar electrokinetic capillary chromatography: development and validation. Electrophoresis, 19(16-17), 2890-4.
Li YM, et al. Analysis of Cefadroxil By Micellar Electrokinetic Capillary Chromatography: Development and Validation. Electrophoresis. 1998;19(16-17):2890-4. PubMed PMID: 9870384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of cefadroxil by micellar electrokinetic capillary chromatography: development and validation. AU - Li,Y M, AU - Vanderghinste,D, AU - Pecanac,D, AU - Van Schepdael,A, AU - Roets,E, AU - Hoogmartens,J, PY - 1998/12/31/pubmed PY - 1998/12/31/medline PY - 1998/12/31/entrez SP - 2890 EP - 4 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 16-17 N2 - A method is developed and validated for analysis of the antibiotic cefadroxil using micellar electrokinetic capillary chromatography. It permits cefadroxil to be completely separated from ten of its known related substances within 15 min (including the washing procedure). The separation is performed in an acetate buffer (50 mM, pH 5.25) containing sodium dodecyl sulfate (SDS; 110 mM). The fused-silica capillary was 44 cm long (36 cm effective length), 50 microm ID; the voltage, 18 kV; temperature, 15 degrees C; and the detection wavelength; 254 nm. The influence of the type of buffer, buffer pH and concentration, and of the SDS concentration was investigated. The robustness of the method was examined by means of a full-fraction factorial design. The parameters for validation such as linearity, precision, limit of detection and limit of quantitation are also reported. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9870384/Analysis_of_cefadroxil_by_micellar_electrokinetic_capillary_chromatography:_development_and_validation_ DB - PRIME DP - Unbound Medicine ER -