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Analysis of fluorescein isothiocyanate derivatized amphetamine and analogs in human urine by capillary electrophoresis in chip-based and fused-silica capillary instrumentation.
Electrophoresis. 1998 Nov; 19(16-17):2967-75.E

Abstract

Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in human urine using chip-based and fused-silica capillary instrumentation with laser-induced fluorescence detection. Data obtained via direct labeling of fortified urine are compared to those generated after FITC labeling of urinary extracts that were prepared by solid-phase extraction using a copolymer phase. For a urine volume of 5 mL with a "spiked amine": FITC ratio of 1:250, the latter approach was found to provide a sensitivity that is relevant for toxicological drug screening and confirmation (about 200 ng/mL urine). With direct labeling of 10 microL urine that was alkalinized and diluted for derivatization, the limit of identification was determined to be about 10 microg/mL, a value that is too high for practical purposes. Compared to fused-silica capillaries, electrophoresis in microstructures is shown to provide faster separations and higher efficiencies without loss of accuracy and precision.

Authors+Show Affiliations

Department of Clinical Pharmacology, University of Bern, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9870397

Citation

Ramseier, A, et al. "Analysis of Fluorescein Isothiocyanate Derivatized Amphetamine and Analogs in Human Urine By Capillary Electrophoresis in Chip-based and Fused-silica Capillary Instrumentation." Electrophoresis, vol. 19, no. 16-17, 1998, pp. 2967-75.
Ramseier A, von Heeren F, Thormann W. Analysis of fluorescein isothiocyanate derivatized amphetamine and analogs in human urine by capillary electrophoresis in chip-based and fused-silica capillary instrumentation. Electrophoresis. 1998;19(16-17):2967-75.
Ramseier, A., von Heeren, F., & Thormann, W. (1998). Analysis of fluorescein isothiocyanate derivatized amphetamine and analogs in human urine by capillary electrophoresis in chip-based and fused-silica capillary instrumentation. Electrophoresis, 19(16-17), 2967-75.
Ramseier A, von Heeren F, Thormann W. Analysis of Fluorescein Isothiocyanate Derivatized Amphetamine and Analogs in Human Urine By Capillary Electrophoresis in Chip-based and Fused-silica Capillary Instrumentation. Electrophoresis. 1998;19(16-17):2967-75. PubMed PMID: 9870397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of fluorescein isothiocyanate derivatized amphetamine and analogs in human urine by capillary electrophoresis in chip-based and fused-silica capillary instrumentation. AU - Ramseier,A, AU - von Heeren,F, AU - Thormann,W, PY - 1998/12/31/pubmed PY - 1998/12/31/medline PY - 1998/12/31/entrez SP - 2967 EP - 75 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 16-17 N2 - Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in human urine using chip-based and fused-silica capillary instrumentation with laser-induced fluorescence detection. Data obtained via direct labeling of fortified urine are compared to those generated after FITC labeling of urinary extracts that were prepared by solid-phase extraction using a copolymer phase. For a urine volume of 5 mL with a "spiked amine": FITC ratio of 1:250, the latter approach was found to provide a sensitivity that is relevant for toxicological drug screening and confirmation (about 200 ng/mL urine). With direct labeling of 10 microL urine that was alkalinized and diluted for derivatization, the limit of identification was determined to be about 10 microg/mL, a value that is too high for practical purposes. Compared to fused-silica capillaries, electrophoresis in microstructures is shown to provide faster separations and higher efficiencies without loss of accuracy and precision. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9870397/Analysis_of_fluorescein_isothiocyanate_derivatized_amphetamine_and_analogs_in_human_urine_by_capillary_electrophoresis_in_chip_based_and_fused_silica_capillary_instrumentation_ L2 - https://doi.org/10.1002/elps.1150191628 DB - PRIME DP - Unbound Medicine ER -