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A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system.
Biochem Biophys Res Commun. 1998 Dec 09; 253(1):65-9.BB

Abstract

The rat insulin promoter (RIP) has been used to drive the expression of Cre recombinase (Cre) specifically in beta cells. Transient transfection was performed in the mouse insulinoma cell line, NIT-1, and control cell lines. RT-PCR was performed using total RNA from pancreas and other tissues of RIP-Cre transgenic mice. In addition, the efficiency and specificity of RIP were further analyzed by cross breeding the RIP-Cre transgenic mice with reporter mice bearing a beta-actin-loxP-CAT-loxP-lacZ transgene. In these mice, lacZ is expressed only after excision of the floxed-CAT gene by Cre-mediated recombination. Here, we present the data for beta cell-specific expression of lacZ in bigenic mice, as proof of concept in a mouse model for targeting beta cell-specific gene(s). The RIP-Cre transgenic mice will be used as a potential tool for targeting the excision of beta cell-specific gene(s) to study their role in islet cell physiology.

Authors+Show Affiliations

Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. mray@bcm.tmc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9875221

Citation

Ray, M K., et al. "A Mouse Model for Beta Cell-specific Ablation of Target Gene(s) Using the Cre-loxP System." Biochemical and Biophysical Research Communications, vol. 253, no. 1, 1998, pp. 65-9.
Ray MK, Fagan SP, Moldovan S, et al. A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system. Biochem Biophys Res Commun. 1998;253(1):65-9.
Ray, M. K., Fagan, S. P., Moldovan, S., DeMayo, F. J., & Brunicardi, F. C. (1998). A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system. Biochemical and Biophysical Research Communications, 253(1), 65-9.
Ray MK, et al. A Mouse Model for Beta Cell-specific Ablation of Target Gene(s) Using the Cre-loxP System. Biochem Biophys Res Commun. 1998 Dec 9;253(1):65-9. PubMed PMID: 9875221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system. AU - Ray,M K, AU - Fagan,S P, AU - Moldovan,S, AU - DeMayo,F J, AU - Brunicardi,F C, PY - 1999/1/6/pubmed PY - 1999/1/6/medline PY - 1999/1/6/entrez SP - 65 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 253 IS - 1 N2 - The rat insulin promoter (RIP) has been used to drive the expression of Cre recombinase (Cre) specifically in beta cells. Transient transfection was performed in the mouse insulinoma cell line, NIT-1, and control cell lines. RT-PCR was performed using total RNA from pancreas and other tissues of RIP-Cre transgenic mice. In addition, the efficiency and specificity of RIP were further analyzed by cross breeding the RIP-Cre transgenic mice with reporter mice bearing a beta-actin-loxP-CAT-loxP-lacZ transgene. In these mice, lacZ is expressed only after excision of the floxed-CAT gene by Cre-mediated recombination. Here, we present the data for beta cell-specific expression of lacZ in bigenic mice, as proof of concept in a mouse model for targeting beta cell-specific gene(s). The RIP-Cre transgenic mice will be used as a potential tool for targeting the excision of beta cell-specific gene(s) to study their role in islet cell physiology. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9875221/A_mouse_model_for_beta_cell_specific_ablation_of_target_gene_s__using_the_Cre_loxP_system_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(98)99714-5 DB - PRIME DP - Unbound Medicine ER -