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Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA.
J Mol Biol. 1998 Dec 18; 284(5):1341-51.JM

Abstract

The structure of the internal transcribed spacer 1 (ITS1) in Schizosaccharomyces pombe was examined with respect to phylogenetically conserved features in yeasts as well as the binding of transacting factors that potentially play a role in ribosomal maturation. Computer analyses and probes for nuclease protection indicate a compact, more highly organized structure than previously proposed in Saccharomyces cerevisiae, with distinct structural features which can be recognized in S. cerevisiae. These include a central extended hairpin structure as well as smaller hairpins immediately adjacent to the maturing termini. Comparisons with ITS sequences in more diverse organisms indicate that the same features also can be recognized. This is especially clear in organisms which contain very short sequences in which the putative structures are much less ambiguous. Again nuclease protection analyses in one of these, Verticillium albo-atrum, confirm a central hairpin with additional hairpins linked to the maturing termini. Protein binding and gel retardation studies with the S. pombe ITS1 further indicate that, as observed in the 3' external transcripted spacer (ETS) region, the extended hairpin is not only the site of intermediate RNA cleavage during rRNA processing, but also a site for specific interactions with one or more soluble factors. Taken together with other analyses on transcribed spacer regions, the present data provide evidence that the spacer regions act not only to organize the maturing terminal sequences but also may serve to organize specific soluble factors, possibly acting in a manner which is analogous with that of the free small nucleolar ribonucleo protein particles (snoRNPs).

Authors+Show Affiliations

Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9878354

Citation

Lalev, A I., and R N. Nazar. "Conserved Core Structure in the Internal Transcribed Spacer 1 of the Schizosaccharomyces Pombe Precursor Ribosomal RNA." Journal of Molecular Biology, vol. 284, no. 5, 1998, pp. 1341-51.
Lalev AI, Nazar RN. Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA. J Mol Biol. 1998;284(5):1341-51.
Lalev, A. I., & Nazar, R. N. (1998). Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA. Journal of Molecular Biology, 284(5), 1341-51.
Lalev AI, Nazar RN. Conserved Core Structure in the Internal Transcribed Spacer 1 of the Schizosaccharomyces Pombe Precursor Ribosomal RNA. J Mol Biol. 1998 Dec 18;284(5):1341-51. PubMed PMID: 9878354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA. AU - Lalev,A I, AU - Nazar,R N, PY - 1999/1/8/pubmed PY - 1999/1/8/medline PY - 1999/1/8/entrez SP - 1341 EP - 51 JF - Journal of molecular biology JO - J Mol Biol VL - 284 IS - 5 N2 - The structure of the internal transcribed spacer 1 (ITS1) in Schizosaccharomyces pombe was examined with respect to phylogenetically conserved features in yeasts as well as the binding of transacting factors that potentially play a role in ribosomal maturation. Computer analyses and probes for nuclease protection indicate a compact, more highly organized structure than previously proposed in Saccharomyces cerevisiae, with distinct structural features which can be recognized in S. cerevisiae. These include a central extended hairpin structure as well as smaller hairpins immediately adjacent to the maturing termini. Comparisons with ITS sequences in more diverse organisms indicate that the same features also can be recognized. This is especially clear in organisms which contain very short sequences in which the putative structures are much less ambiguous. Again nuclease protection analyses in one of these, Verticillium albo-atrum, confirm a central hairpin with additional hairpins linked to the maturing termini. Protein binding and gel retardation studies with the S. pombe ITS1 further indicate that, as observed in the 3' external transcripted spacer (ETS) region, the extended hairpin is not only the site of intermediate RNA cleavage during rRNA processing, but also a site for specific interactions with one or more soluble factors. Taken together with other analyses on transcribed spacer regions, the present data provide evidence that the spacer regions act not only to organize the maturing terminal sequences but also may serve to organize specific soluble factors, possibly acting in a manner which is analogous with that of the free small nucleolar ribonucleo protein particles (snoRNPs). SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/9878354/Conserved_core_structure_in_the_internal_transcribed_spacer_1_of_the_Schizosaccharomyces_pombe_precursor_ribosomal_RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(98)92222-7 DB - PRIME DP - Unbound Medicine ER -