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The PecT repressor coregulates synthesis of exopolysaccharides and virulence factors in Erwinia chrysanthemi.
Mol Plant Microbe Interact. 1999 Jan; 12(1):45-52.MP

Abstract

Erwinia chrysanthemi 3937 synthesizes an exopolysaccharide (EPS) composed of rhamnose, galactose, and galacturonic acid. Fourteen transcriptional fusions in genes required for EPS synthesis, named eps, were obtained by Tn5-B21 mutagenesis. Eleven of them are clustered on the chromosome and are repressed by PecT, a regulator of pectate lyase synthesis. In addition, expression of these fusions is repressed by the catabolite regulatory protein, CRP, and induced in low osmolarity medium. The three other mutations are located in genes that are not regulated by pecT. A 13-kb DNA fragment containing pecT-regulated eps genes has been cloned. All the genes identified on this fragment are transcribed in the same orientation and could form a large operon. The promoter region of this operon has been sequenced. It contains a JUMP-start sequence, a sequence required for the expression of polysaccharide-associated operons. E. chrysanthemi 3937 produces a systemic soft rot on its host Saintpaulia ionantha. An eps mutant was less efficient than the wild-type strain in initiating a maceration symptom, suggesting that production of EPS is required for the full expression of the E. chrysanthemi virulence.

Authors+Show Affiliations

Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, UMR-CNRS 5577, Villeurbanne, France. condemin@insa.insa-lyon.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9885192

Citation

Condemine, G, et al. "The PecT Repressor Coregulates Synthesis of Exopolysaccharides and Virulence Factors in Erwinia Chrysanthemi." Molecular Plant-microbe Interactions : MPMI, vol. 12, no. 1, 1999, pp. 45-52.
Condemine G, Castillo A, Passeri F, et al. The PecT repressor coregulates synthesis of exopolysaccharides and virulence factors in Erwinia chrysanthemi. Mol Plant Microbe Interact. 1999;12(1):45-52.
Condemine, G., Castillo, A., Passeri, F., & Enard, C. (1999). The PecT repressor coregulates synthesis of exopolysaccharides and virulence factors in Erwinia chrysanthemi. Molecular Plant-microbe Interactions : MPMI, 12(1), 45-52.
Condemine G, et al. The PecT Repressor Coregulates Synthesis of Exopolysaccharides and Virulence Factors in Erwinia Chrysanthemi. Mol Plant Microbe Interact. 1999;12(1):45-52. PubMed PMID: 9885192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The PecT repressor coregulates synthesis of exopolysaccharides and virulence factors in Erwinia chrysanthemi. AU - Condemine,G, AU - Castillo,A, AU - Passeri,F, AU - Enard,C, PY - 1999/1/13/pubmed PY - 1999/1/13/medline PY - 1999/1/13/entrez SP - 45 EP - 52 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 12 IS - 1 N2 - Erwinia chrysanthemi 3937 synthesizes an exopolysaccharide (EPS) composed of rhamnose, galactose, and galacturonic acid. Fourteen transcriptional fusions in genes required for EPS synthesis, named eps, were obtained by Tn5-B21 mutagenesis. Eleven of them are clustered on the chromosome and are repressed by PecT, a regulator of pectate lyase synthesis. In addition, expression of these fusions is repressed by the catabolite regulatory protein, CRP, and induced in low osmolarity medium. The three other mutations are located in genes that are not regulated by pecT. A 13-kb DNA fragment containing pecT-regulated eps genes has been cloned. All the genes identified on this fragment are transcribed in the same orientation and could form a large operon. The promoter region of this operon has been sequenced. It contains a JUMP-start sequence, a sequence required for the expression of polysaccharide-associated operons. E. chrysanthemi 3937 produces a systemic soft rot on its host Saintpaulia ionantha. An eps mutant was less efficient than the wild-type strain in initiating a maceration symptom, suggesting that production of EPS is required for the full expression of the E. chrysanthemi virulence. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/9885192/The_PecT_repressor_coregulates_synthesis_of_exopolysaccharides_and_virulence_factors_in_Erwinia_chrysanthemi_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.1.45?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -