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Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy.
Invest Ophthalmol Vis Sci. 1999 Jan; 40(1):149-56.IO

Abstract

PURPOSE

To determine whether hepatocyte growth factor (HGF) receptor (HGFR) is expressed in retinal pigment epithelial (RPE) cells and to test whether RPE cells are responsive to HGF. To evaluate expression of HGFR in human donor eyes and in several epiretinal membranes associated with proliferative vitreoretinopathy and idiopathic epiretinal membranes.

METHODS

HGF-dependent migration and proliferation in primary and simian virus (SV) 40-transformed human RPE cells was studied using a Boyden chamber and [3H]thymidine uptake, respectively. The expression and tyrosine phosphorylation of HGFR protein was evaluated in RPE cells by immunoprecipitation and western blot analysis. Expression of HGFR in human donor eyes and in several epiretinal membranes associated with proliferative vitreoretinopathy (PVR) and idiopathic epiretinal membranes was analyzed by immunohistochemistry.

RESULTS

HGFR was expressed in RPE cells and was tyrosine-phosphorylated in response to HGF. Whereas HGF was a potent motogen for RPE cells, it induced only a modest, dose-dependent uptake of [3H]thymidine. Evaluation of human donor eyes showed that the RPE monolayer was the major cell type that was strongly positive for HGFR. HGFR was uniformly and readily detected in the cellular component of epiretinal membranes associated with PVR, whereas little or no HGFR was found in idiopathic epiretinal membranes.

CONCLUSIONS

HGFR is expressed in cultured RPE cells, in the RPE monolayer in human donor eyes, and in epiretinal membranes obtained from patients with PVR. Furthermore, HGF is a potent chemoattractant for cultured human RPE cells. These observations suggest a role for HGF and HGFR in normal function of RPE cells and in RPE-related disease such as PVR.

Authors+Show Affiliations

The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9888438

Citation

Lashkari, K, et al. "Hepatocyte Growth Factor Receptor in Human RPE Cells: Implications in Proliferative Vitreoretinopathy." Investigative Ophthalmology & Visual Science, vol. 40, no. 1, 1999, pp. 149-56.
Lashkari K, Rahimi N, Kazlauskas A. Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci. 1999;40(1):149-56.
Lashkari, K., Rahimi, N., & Kazlauskas, A. (1999). Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy. Investigative Ophthalmology & Visual Science, 40(1), 149-56.
Lashkari K, Rahimi N, Kazlauskas A. Hepatocyte Growth Factor Receptor in Human RPE Cells: Implications in Proliferative Vitreoretinopathy. Invest Ophthalmol Vis Sci. 1999;40(1):149-56. PubMed PMID: 9888438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy. AU - Lashkari,K, AU - Rahimi,N, AU - Kazlauskas,A, PY - 1999/1/15/pubmed PY - 1999/1/15/medline PY - 1999/1/15/entrez SP - 149 EP - 56 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 40 IS - 1 N2 - PURPOSE: To determine whether hepatocyte growth factor (HGF) receptor (HGFR) is expressed in retinal pigment epithelial (RPE) cells and to test whether RPE cells are responsive to HGF. To evaluate expression of HGFR in human donor eyes and in several epiretinal membranes associated with proliferative vitreoretinopathy and idiopathic epiretinal membranes. METHODS: HGF-dependent migration and proliferation in primary and simian virus (SV) 40-transformed human RPE cells was studied using a Boyden chamber and [3H]thymidine uptake, respectively. The expression and tyrosine phosphorylation of HGFR protein was evaluated in RPE cells by immunoprecipitation and western blot analysis. Expression of HGFR in human donor eyes and in several epiretinal membranes associated with proliferative vitreoretinopathy (PVR) and idiopathic epiretinal membranes was analyzed by immunohistochemistry. RESULTS: HGFR was expressed in RPE cells and was tyrosine-phosphorylated in response to HGF. Whereas HGF was a potent motogen for RPE cells, it induced only a modest, dose-dependent uptake of [3H]thymidine. Evaluation of human donor eyes showed that the RPE monolayer was the major cell type that was strongly positive for HGFR. HGFR was uniformly and readily detected in the cellular component of epiretinal membranes associated with PVR, whereas little or no HGFR was found in idiopathic epiretinal membranes. CONCLUSIONS: HGFR is expressed in cultured RPE cells, in the RPE monolayer in human donor eyes, and in epiretinal membranes obtained from patients with PVR. Furthermore, HGF is a potent chemoattractant for cultured human RPE cells. These observations suggest a role for HGF and HGFR in normal function of RPE cells and in RPE-related disease such as PVR. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/9888438/Hepatocyte_growth_factor_receptor_in_human_RPE_cells:_implications_in_proliferative_vitreoretinopathy_ L2 - http://iovs.arvojournals.org/article.aspx?volume=40&page=149 DB - PRIME DP - Unbound Medicine ER -