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Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma.
J Med Virol. 1999 Jan; 57(1):36-46.JM

Abstract

Cells from a leiomyosarcoma tumor (LMS-1) from a patient with the acquired immunodeficiency syndrome (AIDS) were explanted, cultured in vitro, and studied by phase-contrast microscopy for morphologic and growth characteristics, immunostaining for cell markers, EBER in situ hybridization and polymerase chain reaction for detection of Epstein-Barr virus (EBV), and immunostaining for expression of EBV antigens. The cells exhibited very slow growth in vitro, with unusual elliptical and spindle-shaped morphology and fragmentation of the cytoplasm into long, tapering, cytoplasmic processes. Greater than 90% of cells expressed diffuse distribution of the smooth muscle isoform of actin by immunoperoxidase staining. Approximately 25% of cells expressed very bright fluorescence by immunostaining of the smooth muscle isoforms of calponin and actin. The majority of cells demonstrated a weak signal for CD21; approximately 5-10% of cells showed a strong signal that was confined to cell surfaces. The cultured cells harbored EBV, and infectious EBV continued to be detected by polymerase chain reaction and virus culture through several passages in vitro. Several EBV antigens were expressed, including latent antigen EBNA-1, immediate-early antigen BZLF1, early antigen EA-D, and late antigens, including viral capsid antigen p160, gp125, and membrane antigen gp350. Human umbilical cord lymphocytes that were transformed with virus isolated from cultured cells yielded immortalized cell lines that expressed EBV antigens similar to other EBV-transformed lymphocyte cell lines. These results confirm that EBV is capable of lytic infection of smooth muscle cells with expression of a repertoire of latent and replicative viral products and production of infectious virus. EBV infection of smooth muscle cells may contribute to the oncogenesis of leiomyosarcomas.

Authors+Show Affiliations

Department of Pediatrics, The University of Texas Health Science Center at San Antonio, 78284-7811, USA. jenson@uthscsa.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9890420

Citation

Jenson, H B., et al. "Characterization of Natural Epstein-Barr Virus Infection and Replication in Smooth Muscle Cells From a Leiomyosarcoma." Journal of Medical Virology, vol. 57, no. 1, 1999, pp. 36-46.
Jenson HB, Montalvo EA, McClain KL, et al. Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma. J Med Virol. 1999;57(1):36-46.
Jenson, H. B., Montalvo, E. A., McClain, K. L., Ench, Y., Heard, P., Christy, B. A., Dewalt-Hagan, P. J., & Moyer, M. P. (1999). Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma. Journal of Medical Virology, 57(1), 36-46.
Jenson HB, et al. Characterization of Natural Epstein-Barr Virus Infection and Replication in Smooth Muscle Cells From a Leiomyosarcoma. J Med Virol. 1999;57(1):36-46. PubMed PMID: 9890420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of natural Epstein-Barr virus infection and replication in smooth muscle cells from a leiomyosarcoma. AU - Jenson,H B, AU - Montalvo,E A, AU - McClain,K L, AU - Ench,Y, AU - Heard,P, AU - Christy,B A, AU - Dewalt-Hagan,P J, AU - Moyer,M P, PY - 1999/1/16/pubmed PY - 2000/6/20/medline PY - 1999/1/16/entrez SP - 36 EP - 46 JF - Journal of medical virology JO - J. Med. Virol. VL - 57 IS - 1 N2 - Cells from a leiomyosarcoma tumor (LMS-1) from a patient with the acquired immunodeficiency syndrome (AIDS) were explanted, cultured in vitro, and studied by phase-contrast microscopy for morphologic and growth characteristics, immunostaining for cell markers, EBER in situ hybridization and polymerase chain reaction for detection of Epstein-Barr virus (EBV), and immunostaining for expression of EBV antigens. The cells exhibited very slow growth in vitro, with unusual elliptical and spindle-shaped morphology and fragmentation of the cytoplasm into long, tapering, cytoplasmic processes. Greater than 90% of cells expressed diffuse distribution of the smooth muscle isoform of actin by immunoperoxidase staining. Approximately 25% of cells expressed very bright fluorescence by immunostaining of the smooth muscle isoforms of calponin and actin. The majority of cells demonstrated a weak signal for CD21; approximately 5-10% of cells showed a strong signal that was confined to cell surfaces. The cultured cells harbored EBV, and infectious EBV continued to be detected by polymerase chain reaction and virus culture through several passages in vitro. Several EBV antigens were expressed, including latent antigen EBNA-1, immediate-early antigen BZLF1, early antigen EA-D, and late antigens, including viral capsid antigen p160, gp125, and membrane antigen gp350. Human umbilical cord lymphocytes that were transformed with virus isolated from cultured cells yielded immortalized cell lines that expressed EBV antigens similar to other EBV-transformed lymphocyte cell lines. These results confirm that EBV is capable of lytic infection of smooth muscle cells with expression of a repertoire of latent and replicative viral products and production of infectious virus. EBV infection of smooth muscle cells may contribute to the oncogenesis of leiomyosarcomas. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/9890420/Characterization_of_natural_Epstein_Barr_virus_infection_and_replication_in_smooth_muscle_cells_from_a_leiomyosarcoma_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1999&volume=57&issue=1&spage=36 DB - PRIME DP - Unbound Medicine ER -