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Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast.
Mol Cell Biol. 1999 Feb; 19(2):1144-58.MC

Abstract

Through a computer search of the genome of the yeast Saccharomyces cerevisiae, the coding sequences of seven different box C/D antisense small nucleolar RNAs (snoRNAs) with the structural hallmarks of guides for rRNA ribose methylation have been detected clustered over a 1.4-kb tract in an inter-open reading frame region of chromosome XIII. The corresponding snoRNAs have been positively identified in yeast cells. Disruption of the nonessential snoRNA gene cluster specifically suppressed the seven cognate rRNA ribose methylations but did not result in any growth delay under the conditions of yeast culture tested. The seven snoRNAs are processed from a common polycistronic transcript synthesized from an independent promoter, similar to some plant snoRNAs but in marked contrast with their vertebrate functional homologues processed from pre-mRNA introns containing a single snoRNA. Processing of the polycistronic precursor requires nucleases also involved in rRNA processing, i.e., Rnt1p and Rat1p. After disruption of the RNT1 gene, the yeast ortholog of bacterial RNase III, production of the seven mature snoRNAs was abolished, while the polycistronic snoRNA precursor accumulated. In cells lacking functional Rat1p, an exonuclease involved in the processing of both pre-rRNA and intron-encoded snoRNAs, several processing intermediates of the polycistronic precursor accumulated. This allowed for the mapping in the precursor of the presumptive Rnt1p endonucleolytic cuts which provide entry sites for subsequent exonucleolytic trimming of the pre-snoRNAs. In line with known properties of double-stranded RNA-specific RNase III, pairs of Rnt1p cuts map next to each other on opposite strands of long double-helical stems in the secondary structure predicted for the polycistronic snoRNA precursor.

Authors+Show Affiliations

Biotechnology Research Center, Zhongshan University, Guangzhou 510 275, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9891049

Citation

Qu, L H., et al. "Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed From a Common Polycistronic Transcript By Rat1p and RNase III in Yeast." Molecular and Cellular Biology, vol. 19, no. 2, 1999, pp. 1144-58.
Qu LH, Henras A, Lu YJ, et al. Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast. Mol Cell Biol. 1999;19(2):1144-58.
Qu, L. H., Henras, A., Lu, Y. J., Zhou, H., Zhou, W. X., Zhu, Y. Q., Zhao, J., Henry, Y., Caizergues-Ferrer, M., & Bachellerie, J. P. (1999). Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast. Molecular and Cellular Biology, 19(2), 1144-58.
Qu LH, et al. Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed From a Common Polycistronic Transcript By Rat1p and RNase III in Yeast. Mol Cell Biol. 1999;19(2):1144-58. PubMed PMID: 9891049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast. AU - Qu,L H, AU - Henras,A, AU - Lu,Y J, AU - Zhou,H, AU - Zhou,W X, AU - Zhu,Y Q, AU - Zhao,J, AU - Henry,Y, AU - Caizergues-Ferrer,M, AU - Bachellerie,J P, PY - 1999/1/16/pubmed PY - 1999/1/16/medline PY - 1999/1/16/entrez SP - 1144 EP - 58 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 19 IS - 2 N2 - Through a computer search of the genome of the yeast Saccharomyces cerevisiae, the coding sequences of seven different box C/D antisense small nucleolar RNAs (snoRNAs) with the structural hallmarks of guides for rRNA ribose methylation have been detected clustered over a 1.4-kb tract in an inter-open reading frame region of chromosome XIII. The corresponding snoRNAs have been positively identified in yeast cells. Disruption of the nonessential snoRNA gene cluster specifically suppressed the seven cognate rRNA ribose methylations but did not result in any growth delay under the conditions of yeast culture tested. The seven snoRNAs are processed from a common polycistronic transcript synthesized from an independent promoter, similar to some plant snoRNAs but in marked contrast with their vertebrate functional homologues processed from pre-mRNA introns containing a single snoRNA. Processing of the polycistronic precursor requires nucleases also involved in rRNA processing, i.e., Rnt1p and Rat1p. After disruption of the RNT1 gene, the yeast ortholog of bacterial RNase III, production of the seven mature snoRNAs was abolished, while the polycistronic snoRNA precursor accumulated. In cells lacking functional Rat1p, an exonuclease involved in the processing of both pre-rRNA and intron-encoded snoRNAs, several processing intermediates of the polycistronic precursor accumulated. This allowed for the mapping in the precursor of the presumptive Rnt1p endonucleolytic cuts which provide entry sites for subsequent exonucleolytic trimming of the pre-snoRNAs. In line with known properties of double-stranded RNA-specific RNase III, pairs of Rnt1p cuts map next to each other on opposite strands of long double-helical stems in the secondary structure predicted for the polycistronic snoRNA precursor. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/9891049/Seven_novel_methylation_guide_small_nucleolar_RNAs_are_processed_from_a_common_polycistronic_transcript_by_Rat1p_and_RNase_III_in_yeast_ L2 - https://journals.asm.org/doi/10.1128/MCB.19.2.1144?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -