Tags

Type your tag names separated by a space and hit enter

Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA.
J Med Virol. 1999 Feb; 57(2):104-10.JM

Abstract

The sequence encoding the truncated core protein (amino acids 1-98) of hepatitis C virus (HCc) was expressed in E. coli for production of HCc(1-98), or fused with the truncated core antigen (HBcAg) and segments from the preS1 and preS2 regions from hepatitis B virus (HBV) for production of HBcPreS1PreS2HCc(1-98). The HCc(1-98) and HBcPreS1PreS2HCc(1-98) proteins reacted with sera from HCV-infected individuals by immunoblot analyses, while the latter protein also exhibited HBV core antigenicity. They induced antibodies against HBcAg and/or HCV core protein in rabbits and in mice. Moreover, HBcPreS1PreS2HCc(1-98) is more immunogenic than HCc(1-98) in terms of anti-HCc induction. An ELISA that employed recombinant HCV core antigens of either HCc(1-98) or HBcPreS1PreS2HCc(1-98) to detect anti-HCc and/or anti-HBc antibodies was developed. Evaluation of serum samples with different status of HBV and HCV infections suggested that HCc(1-98) might be suitable for the determination of antibodies against HCV core protein, while HBcPreS1PreS2HCc(1-98) might be of value to detect HCV and/or HBV infection in donated blood in HBV low-prevalence countries.

Authors+Show Affiliations

Department of Biochemistry, National Cheng Kung University Medical College, Tainan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9892392

Citation

Wu, C L., et al. "Hepatitis C Virus Core Protein Fused to Hepatitis B Virus Core Antigen for Serological Diagnosis of Both Hepatitis C and Hepatitis B Infections By ELISA." Journal of Medical Virology, vol. 57, no. 2, 1999, pp. 104-10.
Wu CL, Leu TS, Chang TT, et al. Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA. J Med Virol. 1999;57(2):104-10.
Wu, C. L., Leu, T. S., Chang, T. T., & Shiau, A. L. (1999). Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA. Journal of Medical Virology, 57(2), 104-10.
Wu CL, et al. Hepatitis C Virus Core Protein Fused to Hepatitis B Virus Core Antigen for Serological Diagnosis of Both Hepatitis C and Hepatitis B Infections By ELISA. J Med Virol. 1999;57(2):104-10. PubMed PMID: 9892392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA. AU - Wu,C L, AU - Leu,T S, AU - Chang,T T, AU - Shiau,A L, PY - 1999/1/19/pubmed PY - 2000/6/20/medline PY - 1999/1/19/entrez SP - 104 EP - 10 JF - Journal of medical virology JO - J. Med. Virol. VL - 57 IS - 2 N2 - The sequence encoding the truncated core protein (amino acids 1-98) of hepatitis C virus (HCc) was expressed in E. coli for production of HCc(1-98), or fused with the truncated core antigen (HBcAg) and segments from the preS1 and preS2 regions from hepatitis B virus (HBV) for production of HBcPreS1PreS2HCc(1-98). The HCc(1-98) and HBcPreS1PreS2HCc(1-98) proteins reacted with sera from HCV-infected individuals by immunoblot analyses, while the latter protein also exhibited HBV core antigenicity. They induced antibodies against HBcAg and/or HCV core protein in rabbits and in mice. Moreover, HBcPreS1PreS2HCc(1-98) is more immunogenic than HCc(1-98) in terms of anti-HCc induction. An ELISA that employed recombinant HCV core antigens of either HCc(1-98) or HBcPreS1PreS2HCc(1-98) to detect anti-HCc and/or anti-HBc antibodies was developed. Evaluation of serum samples with different status of HBV and HCV infections suggested that HCc(1-98) might be suitable for the determination of antibodies against HCV core protein, while HBcPreS1PreS2HCc(1-98) might be of value to detect HCV and/or HBV infection in donated blood in HBV low-prevalence countries. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/9892392/Hepatitis_C_virus_core_protein_fused_to_hepatitis_B_virus_core_antigen_for_serological_diagnosis_of_both_hepatitis_C_and_hepatitis_B_infections_by_ELISA_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1999&volume=57&issue=2&spage=104 DB - PRIME DP - Unbound Medicine ER -