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Effects of R and S enantiomers and a racemic mixture of carprofen on the production and release of proteoglycan and prostaglandin E2 from equine chondrocytes and cartilage explants.
Am J Vet Res. 1999 Jan; 60(1):98-104.AJ

Abstract

OBJECTIVE

To examine effects of carprofen (enantiomers and a racemic mixture) on the metabolism of equine chondrocytes.

SAMPLE POPULATION

Cartilage from clinically normal horses.

PROCEDURE

Effects of carprofen on proteoglycan neosynthesis, glycosaminoglycan (GAG) release and prostaglandin (PG) E2 production by unstimulated chondrocyte monolayers and cartilage explants were examined, as were similar variables in monolayers and explants exposed to carprofen and recombinant human interleukin 1beta (IL-1). Carprofen (enantiomers and racemic mixture) was used alone or along with IL-1 on monolayers and explant cultures. Medium was collected 48 to 96 hours later, and cartilage was digested. Proteoglycan synthesis was assessed as the amount of 35S-labeled proteoglycan in medium and digested cartilage. Total GAG content of the medium and digested cartilage was measured, and proteoglycan degradation was calculated. Radioimmunoassay was used to measure PGE2 production.

RESULTS

Carprofen significantly decreased PGE2 production by unstimulated chondrocytes and antagonized an IL-1-induced increase in PGE2 production. Carprofen significantly increased proteoglycan synthesis in unstimulated monolayers and explants. Concurrently, there was a decrease in GAG release by explants. Use of IL-1 significantly decreased proteoglycan synthesis, but the highest concentrations of carprofen partially reversed this effect in chondrocyte monolayers.

CONCLUSIONS

Carprofen had a potentially beneficial effect on proteoglycan metabolism of equine chondrocytes. This effect was sufficiently strong at the highest concentrations to overcome inhibitory effects of IL-1 on proteoglycan synthesis. Carprofen also inhibited PGE2 production by unstimulated and IL-1-stimulated chondrocytes. Carprofen induced these enantiomer-specific effects.

CLINICAL RELEVANCE

Use of carprofen in osteoarthritic horses may induce beneficial changes in articular cartilage matrix.

Authors+Show Affiliations

Department of Veterinary Basic Sciences, Royal Veterinary College, Hertfordshire, United Kingdom.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9918155

Citation

Armstrong, S, and P Lees. "Effects of R and S Enantiomers and a Racemic Mixture of Carprofen On the Production and Release of Proteoglycan and Prostaglandin E2 From Equine Chondrocytes and Cartilage Explants." American Journal of Veterinary Research, vol. 60, no. 1, 1999, pp. 98-104.
Armstrong S, Lees P. Effects of R and S enantiomers and a racemic mixture of carprofen on the production and release of proteoglycan and prostaglandin E2 from equine chondrocytes and cartilage explants. Am J Vet Res. 1999;60(1):98-104.
Armstrong, S., & Lees, P. (1999). Effects of R and S enantiomers and a racemic mixture of carprofen on the production and release of proteoglycan and prostaglandin E2 from equine chondrocytes and cartilage explants. American Journal of Veterinary Research, 60(1), 98-104.
Armstrong S, Lees P. Effects of R and S Enantiomers and a Racemic Mixture of Carprofen On the Production and Release of Proteoglycan and Prostaglandin E2 From Equine Chondrocytes and Cartilage Explants. Am J Vet Res. 1999;60(1):98-104. PubMed PMID: 9918155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of R and S enantiomers and a racemic mixture of carprofen on the production and release of proteoglycan and prostaglandin E2 from equine chondrocytes and cartilage explants. AU - Armstrong,S, AU - Lees,P, PY - 1999/1/26/pubmed PY - 1999/1/26/medline PY - 1999/1/26/entrez SP - 98 EP - 104 JF - American journal of veterinary research JO - Am. J. Vet. Res. VL - 60 IS - 1 N2 - OBJECTIVE: To examine effects of carprofen (enantiomers and a racemic mixture) on the metabolism of equine chondrocytes. SAMPLE POPULATION: Cartilage from clinically normal horses. PROCEDURE: Effects of carprofen on proteoglycan neosynthesis, glycosaminoglycan (GAG) release and prostaglandin (PG) E2 production by unstimulated chondrocyte monolayers and cartilage explants were examined, as were similar variables in monolayers and explants exposed to carprofen and recombinant human interleukin 1beta (IL-1). Carprofen (enantiomers and racemic mixture) was used alone or along with IL-1 on monolayers and explant cultures. Medium was collected 48 to 96 hours later, and cartilage was digested. Proteoglycan synthesis was assessed as the amount of 35S-labeled proteoglycan in medium and digested cartilage. Total GAG content of the medium and digested cartilage was measured, and proteoglycan degradation was calculated. Radioimmunoassay was used to measure PGE2 production. RESULTS: Carprofen significantly decreased PGE2 production by unstimulated chondrocytes and antagonized an IL-1-induced increase in PGE2 production. Carprofen significantly increased proteoglycan synthesis in unstimulated monolayers and explants. Concurrently, there was a decrease in GAG release by explants. Use of IL-1 significantly decreased proteoglycan synthesis, but the highest concentrations of carprofen partially reversed this effect in chondrocyte monolayers. CONCLUSIONS: Carprofen had a potentially beneficial effect on proteoglycan metabolism of equine chondrocytes. This effect was sufficiently strong at the highest concentrations to overcome inhibitory effects of IL-1 on proteoglycan synthesis. Carprofen also inhibited PGE2 production by unstimulated and IL-1-stimulated chondrocytes. Carprofen induced these enantiomer-specific effects. CLINICAL RELEVANCE: Use of carprofen in osteoarthritic horses may induce beneficial changes in articular cartilage matrix. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/9918155/Effects_of_R_and_S_enantiomers_and_a_racemic_mixture_of_carprofen_on_the_production_and_release_of_proteoglycan_and_prostaglandin_E2_from_equine_chondrocytes_and_cartilage_explants_ L2 - https://www.lens.org/lens/search?q=citation_id:9918155 DB - PRIME DP - Unbound Medicine ER -