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Expression pattern of gastrointestinal selenoproteins--targets for selenium supplementation.
Nutr Cancer 1998; 32(2):64-70NC

Abstract

There is experimental and epidemiological evidence for an association between low selenium levels and gastrointestinal cancer incidence, prevalence, and mortality. To identify targets for selenium supplementation in the human digestive tract, we examined mRNA expression of various selenocysteine-containing proteins in normal mucosa biopsy specimens. Tissue samples from the esophagus and from different sites of the stomach, small bowel, and colon were obtained during endoscopies of the upper and lower gastrointestinal tract. Northern blot analyses revealed a lack of cytosolic glutathione peroxidase mRNA but a differential mRNA expression pattern of gastrointestinal and plasma glutathione peroxidase, selenoprotein P, and thioredoxin reductase. Glutathione peroxidase and thioredoxin reductase activities were detected in the mucosa of all biopsies, but the differential pattern did not reflect the differential mRNA steady-state levels. In addition to gastrointestinal glutathione peroxidase, which was found to play a role in colon cancer resistance, we identified further gastrointestinal selenoproteins, which may be involved in gastrointestinal cell defense and cell differentiation.

Authors+Show Affiliations

Medizinische Poliklinik/Klinische Forschergruppe, Universität Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9919613

Citation

Mörk, H, et al. "Expression Pattern of Gastrointestinal Selenoproteins--targets for Selenium Supplementation." Nutrition and Cancer, vol. 32, no. 2, 1998, pp. 64-70.
Mörk H, Lex B, Scheurlen M, et al. Expression pattern of gastrointestinal selenoproteins--targets for selenium supplementation. Nutr Cancer. 1998;32(2):64-70.
Mörk, H., Lex, B., Scheurlen, M., Dreher, I., Schütze, N., Köhrle, J., & Jakob, F. (1998). Expression pattern of gastrointestinal selenoproteins--targets for selenium supplementation. Nutrition and Cancer, 32(2), pp. 64-70.
Mörk H, et al. Expression Pattern of Gastrointestinal Selenoproteins--targets for Selenium Supplementation. Nutr Cancer. 1998;32(2):64-70. PubMed PMID: 9919613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression pattern of gastrointestinal selenoproteins--targets for selenium supplementation. AU - Mörk,H, AU - Lex,B, AU - Scheurlen,M, AU - Dreher,I, AU - Schütze,N, AU - Köhrle,J, AU - Jakob,F, PY - 1999/1/27/pubmed PY - 1999/1/27/medline PY - 1999/1/27/entrez SP - 64 EP - 70 JF - Nutrition and cancer JO - Nutr Cancer VL - 32 IS - 2 N2 - There is experimental and epidemiological evidence for an association between low selenium levels and gastrointestinal cancer incidence, prevalence, and mortality. To identify targets for selenium supplementation in the human digestive tract, we examined mRNA expression of various selenocysteine-containing proteins in normal mucosa biopsy specimens. Tissue samples from the esophagus and from different sites of the stomach, small bowel, and colon were obtained during endoscopies of the upper and lower gastrointestinal tract. Northern blot analyses revealed a lack of cytosolic glutathione peroxidase mRNA but a differential mRNA expression pattern of gastrointestinal and plasma glutathione peroxidase, selenoprotein P, and thioredoxin reductase. Glutathione peroxidase and thioredoxin reductase activities were detected in the mucosa of all biopsies, but the differential pattern did not reflect the differential mRNA steady-state levels. In addition to gastrointestinal glutathione peroxidase, which was found to play a role in colon cancer resistance, we identified further gastrointestinal selenoproteins, which may be involved in gastrointestinal cell defense and cell differentiation. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/9919613/Expression_pattern_of_gastrointestinal_selenoproteins__targets_for_selenium_supplementation_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635589809514720 DB - PRIME DP - Unbound Medicine ER -