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Radiation sensitivity of an Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase.
Biochem Biophys Res Commun. 1999 Jan 27; 254(3):647-50.BB

Abstract

Ionizing radiation induces the production of reactive oxygen species, which play an important causative role in radiation damage. NADP+-dependent isocitrate dehydrogenase (ICDH) in Escherichia coli produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against ionizing radiation in E. coli was investigated in wild-type and ICDH-deficient strains. Upon exposure to ionizing radiation, the viability was lower and the lipid peroxidation was higher in mutant cells compared to wild-type cells. Activities of key antioxidant enzymes such as superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase were decreased by irradiation in both cells. Results suggest that ICDH plays an important role as an antioxidant enzyme in cellular defense against ionizing radiation.

Authors+Show Affiliations

Department of Biochemistry, Kyungpook National University, Taegu, 702-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9920794

Citation

Lee, S M., et al. "Radiation Sensitivity of an Escherichia Coli Mutant Lacking NADP+-dependent Isocitrate Dehydrogenase." Biochemical and Biophysical Research Communications, vol. 254, no. 3, 1999, pp. 647-50.
Lee SM, Koh HJ, Huh TL, et al. Radiation sensitivity of an Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. Biochem Biophys Res Commun. 1999;254(3):647-50.
Lee, S. M., Koh, H. J., Huh, T. L., & Park, J. W. (1999). Radiation sensitivity of an Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. Biochemical and Biophysical Research Communications, 254(3), 647-50.
Lee SM, et al. Radiation Sensitivity of an Escherichia Coli Mutant Lacking NADP+-dependent Isocitrate Dehydrogenase. Biochem Biophys Res Commun. 1999 Jan 27;254(3):647-50. PubMed PMID: 9920794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Radiation sensitivity of an Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. AU - Lee,S M, AU - Koh,H J, AU - Huh,T L, AU - Park,J W, PY - 1999/1/28/pubmed PY - 1999/1/28/medline PY - 1999/1/28/entrez SP - 647 EP - 50 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 254 IS - 3 N2 - Ionizing radiation induces the production of reactive oxygen species, which play an important causative role in radiation damage. NADP+-dependent isocitrate dehydrogenase (ICDH) in Escherichia coli produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against ionizing radiation in E. coli was investigated in wild-type and ICDH-deficient strains. Upon exposure to ionizing radiation, the viability was lower and the lipid peroxidation was higher in mutant cells compared to wild-type cells. Activities of key antioxidant enzymes such as superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase were decreased by irradiation in both cells. Results suggest that ICDH plays an important role as an antioxidant enzyme in cellular defense against ionizing radiation. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9920794/Radiation_sensitivity_of_an_Escherichia_coli_mutant_lacking_NADP+_dependent_isocitrate_dehydrogenase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(98)90125-5 DB - PRIME DP - Unbound Medicine ER -