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HPLC/tandem electrospray mass spectrometry for the determination of Sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish.
Anal Chem. 1999 Jan 01; 71(1):247-50.AC

Abstract

Ciguatera is a significant food-borne disease caused by potent polyether toxins (ciguatoxins) which accumulate in the flesh of ciguateric reef fish at risk levels > 0.1 ppb for Pacific ciguatoxins. Research on ciguatera has been severely hindered by the lack of analytical methods that detect and characterize low levels of ciguatoxin in crude extracts of fish. Here we report a new procedure for ciguatoxin analysis based on gradient reversed-phase HPLC/tandem mass spectrometry (HPLC/MS/MS). The method gave a linear response to pure Pacific and Caribbean ciguatoxins (P-CTX-1 and C-CTX-1) and the structurally related brevetoxin (PbTx-2) spiked into crude extracts of fish. Levels equivalent to 40 ppt P-CTX-1, 100 ppt C-CTX-1, and 200 ppt PbTx-2 in fish flesh could be detected by HPLC/MS/MS. Using P-CTX-1 as an internal standard, the analysis of extracts of 30 ciguateric fish from the Caribbean Sea (8 toxic, 12 borderline, and 10 nontoxic by mouse bioassay) confirmed the reliability of the method and allowed an estimated risk level of > 0.25 ppb C-CTX-1 to be established. HPLC/MS/MS provides a sensitive analytical approach, not previously available, for the determination of Pacific and Caribbean ciguatoxins at sub-ppb levels in fish flesh.

Authors+Show Affiliations

Queensland Agricultural Biotechnology Centre (QDPI), University of Queensland, Australia. r.lewis@mailbox.uq.edu.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9921131

Citation

Lewis, R J., et al. "HPLC/tandem Electrospray Mass Spectrometry for the Determination of Sub-ppb Levels of Pacific and Caribbean Ciguatoxins in Crude Extracts of Fish." Analytical Chemistry, vol. 71, no. 1, 1999, pp. 247-50.
Lewis RJ, Jones A, Vernoux JP. HPLC/tandem electrospray mass spectrometry for the determination of Sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish. Anal Chem. 1999;71(1):247-50.
Lewis, R. J., Jones, A., & Vernoux, J. P. (1999). HPLC/tandem electrospray mass spectrometry for the determination of Sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish. Analytical Chemistry, 71(1), 247-50.
Lewis RJ, Jones A, Vernoux JP. HPLC/tandem Electrospray Mass Spectrometry for the Determination of Sub-ppb Levels of Pacific and Caribbean Ciguatoxins in Crude Extracts of Fish. Anal Chem. 1999 Jan 1;71(1):247-50. PubMed PMID: 9921131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC/tandem electrospray mass spectrometry for the determination of Sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish. AU - Lewis,R J, AU - Jones,A, AU - Vernoux,J P, PY - 1999/1/28/pubmed PY - 1999/1/28/medline PY - 1999/1/28/entrez SP - 247 EP - 50 JF - Analytical chemistry JO - Anal Chem VL - 71 IS - 1 N2 - Ciguatera is a significant food-borne disease caused by potent polyether toxins (ciguatoxins) which accumulate in the flesh of ciguateric reef fish at risk levels > 0.1 ppb for Pacific ciguatoxins. Research on ciguatera has been severely hindered by the lack of analytical methods that detect and characterize low levels of ciguatoxin in crude extracts of fish. Here we report a new procedure for ciguatoxin analysis based on gradient reversed-phase HPLC/tandem mass spectrometry (HPLC/MS/MS). The method gave a linear response to pure Pacific and Caribbean ciguatoxins (P-CTX-1 and C-CTX-1) and the structurally related brevetoxin (PbTx-2) spiked into crude extracts of fish. Levels equivalent to 40 ppt P-CTX-1, 100 ppt C-CTX-1, and 200 ppt PbTx-2 in fish flesh could be detected by HPLC/MS/MS. Using P-CTX-1 as an internal standard, the analysis of extracts of 30 ciguateric fish from the Caribbean Sea (8 toxic, 12 borderline, and 10 nontoxic by mouse bioassay) confirmed the reliability of the method and allowed an estimated risk level of > 0.25 ppb C-CTX-1 to be established. HPLC/MS/MS provides a sensitive analytical approach, not previously available, for the determination of Pacific and Caribbean ciguatoxins at sub-ppb levels in fish flesh. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/9921131/HPLC/tandem_electrospray_mass_spectrometry_for_the_determination_of_Sub_ppb_levels_of_Pacific_and_Caribbean_ciguatoxins_in_crude_extracts_of_fish_ L2 - https://doi.org/10.1021/ac980598h DB - PRIME DP - Unbound Medicine ER -