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Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997-1998.
Virology 1999; 254(1):115-23V

Abstract

Influenza A (H5N1) viruses infected humans in Hong Kong between May and December, 1997. Sixteen viruses, including 6 from fatal cases, were isolated during this outbreak. Molecular analysis of the surface proteins genes encoding the hemagglutinin (HA) and neuraminidase (NA) of these H5N1 isolates, of a subtype not previously known to infect humans, are presented. The 16 human H5 HA sequences contain multiple basic amino acids adjacent to the cleavage site, a motif associated with highly pathogenic avian influenza A viruses. The phylogenetic relationship among both avian and human H5 hemagglutinins indicates that the human isolates are related directly to isolates that circulated among chickens in the live poultry markets in Hong Kong prior to and during the outbreak in humans. HA sequences from the human isolates and a recent chicken isolate represent a separate clade, within which there are two subgroups that are distinguishable antigenically and by the presence of a potential glycosylation site. Likewise the N1 neuraminidases of the human H5 isolates represent a clade that is evolutionarily distinct from previously characterized N1 neuraminidases. The recent human H5N1 virus NA genes are avian-like, indicating direct introduction from an avian source rather than evolution of a human N1 NA. All of the 16 human NA genes encode a shortened stalk due to a 19-amino acid deletion, also found in the recent avian H5N1 isolates from Hong Kong. Two unique amino acids were identified in the N1 NAs of the recent human isolates; however, it is not known if these residues influence host range. Neither the HA nor the NA genes of the human H5N1 virus isolates show evidence of adaptive changes during the outbreak. Although analyses of the surface protein genes of the H5N1 viruses from this outbreak did not provide immediate answers regarding the molecular basis for virulence, the analyses provided clues to potentially important areas of the genes worth further investigation.

Authors+Show Affiliations

Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, 30333, USA. cab2@cdc.gov

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9927579

Citation

Bender, C, et al. "Characterization of the Surface Proteins of Influenza a (H5N1) Viruses Isolated From Humans in 1997-1998." Virology, vol. 254, no. 1, 1999, pp. 115-23.
Bender C, Hall H, Huang J, et al. Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997-1998. Virology. 1999;254(1):115-23.
Bender, C., Hall, H., Huang, J., Klimov, A., Cox, N., Hay, A., ... Subbarao, K. (1999). Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997-1998. Virology, 254(1), pp. 115-23.
Bender C, et al. Characterization of the Surface Proteins of Influenza a (H5N1) Viruses Isolated From Humans in 1997-1998. Virology. 1999 Feb 1;254(1):115-23. PubMed PMID: 9927579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997-1998. AU - Bender,C, AU - Hall,H, AU - Huang,J, AU - Klimov,A, AU - Cox,N, AU - Hay,A, AU - Gregory,V, AU - Cameron,K, AU - Lim,W, AU - Subbarao,K, PY - 1999/2/3/pubmed PY - 1999/2/3/medline PY - 1999/2/3/entrez SP - 115 EP - 23 JF - Virology JO - Virology VL - 254 IS - 1 N2 - Influenza A (H5N1) viruses infected humans in Hong Kong between May and December, 1997. Sixteen viruses, including 6 from fatal cases, were isolated during this outbreak. Molecular analysis of the surface proteins genes encoding the hemagglutinin (HA) and neuraminidase (NA) of these H5N1 isolates, of a subtype not previously known to infect humans, are presented. The 16 human H5 HA sequences contain multiple basic amino acids adjacent to the cleavage site, a motif associated with highly pathogenic avian influenza A viruses. The phylogenetic relationship among both avian and human H5 hemagglutinins indicates that the human isolates are related directly to isolates that circulated among chickens in the live poultry markets in Hong Kong prior to and during the outbreak in humans. HA sequences from the human isolates and a recent chicken isolate represent a separate clade, within which there are two subgroups that are distinguishable antigenically and by the presence of a potential glycosylation site. Likewise the N1 neuraminidases of the human H5 isolates represent a clade that is evolutionarily distinct from previously characterized N1 neuraminidases. The recent human H5N1 virus NA genes are avian-like, indicating direct introduction from an avian source rather than evolution of a human N1 NA. All of the 16 human NA genes encode a shortened stalk due to a 19-amino acid deletion, also found in the recent avian H5N1 isolates from Hong Kong. Two unique amino acids were identified in the N1 NAs of the recent human isolates; however, it is not known if these residues influence host range. Neither the HA nor the NA genes of the human H5N1 virus isolates show evidence of adaptive changes during the outbreak. Although analyses of the surface protein genes of the H5N1 viruses from this outbreak did not provide immediate answers regarding the molecular basis for virulence, the analyses provided clues to potentially important areas of the genes worth further investigation. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/9927579/Characterization_of_the_surface_proteins_of_influenza_A__H5N1__viruses_isolated_from_humans_in_1997_1998_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(98)99529-0 DB - PRIME DP - Unbound Medicine ER -