Tags

Type your tag names separated by a space and hit enter

The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching.
Mol Gen Genet. 1999 Jan; 260(6):551-8.MG

Abstract

Homothallic Saccharomyces cerevisiae strains switch their mating-type in a specific gene conversion event induced by a DNA double strand break made by the HO endonuclease. The RAD52 group genes control recombinational repair of DNA double strand breaks, and we examined their role in native homothallic mating-type switching. Surprisingly, we found that the Rad54 protein was important but not essential for mating-type switching under natural conditions. As an upper limit, we estimate that 29% of the rad54 spore clones can successfully switch their mating-type. The RAD55 and RAD57 gene products were even less important, but their presence increased the efficiency of the process. In contrast, the RAD51 and RAD52 genes are essential for homothallic mating-type switching. We propose that mating-type switching in RAD54 mutants occurs stochastically with a low probability, possibly reflecting different states of chromosomal structure.

Authors+Show Affiliations

Institute of General Microbiology, Bern, Switzerland.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9928934

Citation

Schmuckli-Maurer, J, and W D. Heyer. "The Saccharomyces Cerevisiae RAD54 Gene Is Important but Not Essential for Natural Homothallic Mating-type Switching." Molecular & General Genetics : MGG, vol. 260, no. 6, 1999, pp. 551-8.
Schmuckli-Maurer J, Heyer WD. The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching. Mol Gen Genet. 1999;260(6):551-8.
Schmuckli-Maurer, J., & Heyer, W. D. (1999). The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching. Molecular & General Genetics : MGG, 260(6), 551-8.
Schmuckli-Maurer J, Heyer WD. The Saccharomyces Cerevisiae RAD54 Gene Is Important but Not Essential for Natural Homothallic Mating-type Switching. Mol Gen Genet. 1999;260(6):551-8. PubMed PMID: 9928934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching. AU - Schmuckli-Maurer,J, AU - Heyer,W D, PY - 1999/2/3/pubmed PY - 1999/2/3/medline PY - 1999/2/3/entrez SP - 551 EP - 8 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 260 IS - 6 N2 - Homothallic Saccharomyces cerevisiae strains switch their mating-type in a specific gene conversion event induced by a DNA double strand break made by the HO endonuclease. The RAD52 group genes control recombinational repair of DNA double strand breaks, and we examined their role in native homothallic mating-type switching. Surprisingly, we found that the Rad54 protein was important but not essential for mating-type switching under natural conditions. As an upper limit, we estimate that 29% of the rad54 spore clones can successfully switch their mating-type. The RAD55 and RAD57 gene products were even less important, but their presence increased the efficiency of the process. In contrast, the RAD51 and RAD52 genes are essential for homothallic mating-type switching. We propose that mating-type switching in RAD54 mutants occurs stochastically with a low probability, possibly reflecting different states of chromosomal structure. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/9928934/The_Saccharomyces_cerevisiae_RAD54_gene_is_important_but_not_essential_for_natural_homothallic_mating_type_switching_ L2 - https://doi.org/10.1007/s004380050928 DB - PRIME DP - Unbound Medicine ER -