Tags

Type your tag names separated by a space and hit enter

Excitotoxic death of a subset of embryonic rat motor neurons in vitro.
J Neurochem. 1999 Feb; 72(2):500-13.JN

Abstract

We have used cultures of purified embryonic rat spinal cord motor neurons to study the neurotoxic effects of prolonged ionotropic glutamate receptor activation. NMDA and non-NMDA glutamate receptor agonists kill a maximum of 40% of the motor neurons in a concentration- and time-dependent manner, which can be blocked by receptor subtype-specific antagonists. Subunit-specific antibodies stain all of the motor neurons with approximately the same intensity and for the same repertoire of subunits, suggesting that the survival of the nonvulnerable population is unlikely to be due to the lack of glutamate receptor expression. Extracellular Ca2+ is required for excitotoxicity, and the route of entry initiated by activation of non-NMDA, but not NMDA, receptors is L-type Ca2+ channels. Ca2+ imaging of motor neurons after application of specific glutamate receptor agonists reveals a sustained rise in intracellular Ca2+ that is present to a similar degree in most motor neurons, and can be blocked by appropriate receptor/channel antagonists. Although the lethal effects of glutamate receptor agonists are seen in only a subset of cultured motor neurons, the basis of this selectivity is unlikely to be simply the glutamate receptor phenotype or the level/pattern of rise in agonist-evoked intracellular Ca2+.

Authors+Show Affiliations

Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520-8018, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9930721

Citation

Fryer, H J., et al. "Excitotoxic Death of a Subset of Embryonic Rat Motor Neurons in Vitro." Journal of Neurochemistry, vol. 72, no. 2, 1999, pp. 500-13.
Fryer HJ, Knox RJ, Strittmatter SM, et al. Excitotoxic death of a subset of embryonic rat motor neurons in vitro. J Neurochem. 1999;72(2):500-13.
Fryer, H. J., Knox, R. J., Strittmatter, S. M., & Kalb, R. G. (1999). Excitotoxic death of a subset of embryonic rat motor neurons in vitro. Journal of Neurochemistry, 72(2), 500-13.
Fryer HJ, et al. Excitotoxic Death of a Subset of Embryonic Rat Motor Neurons in Vitro. J Neurochem. 1999;72(2):500-13. PubMed PMID: 9930721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excitotoxic death of a subset of embryonic rat motor neurons in vitro. AU - Fryer,H J, AU - Knox,R J, AU - Strittmatter,S M, AU - Kalb,R G, PY - 1999/2/4/pubmed PY - 1999/2/4/medline PY - 1999/2/4/entrez SP - 500 EP - 13 JF - Journal of neurochemistry JO - J Neurochem VL - 72 IS - 2 N2 - We have used cultures of purified embryonic rat spinal cord motor neurons to study the neurotoxic effects of prolonged ionotropic glutamate receptor activation. NMDA and non-NMDA glutamate receptor agonists kill a maximum of 40% of the motor neurons in a concentration- and time-dependent manner, which can be blocked by receptor subtype-specific antagonists. Subunit-specific antibodies stain all of the motor neurons with approximately the same intensity and for the same repertoire of subunits, suggesting that the survival of the nonvulnerable population is unlikely to be due to the lack of glutamate receptor expression. Extracellular Ca2+ is required for excitotoxicity, and the route of entry initiated by activation of non-NMDA, but not NMDA, receptors is L-type Ca2+ channels. Ca2+ imaging of motor neurons after application of specific glutamate receptor agonists reveals a sustained rise in intracellular Ca2+ that is present to a similar degree in most motor neurons, and can be blocked by appropriate receptor/channel antagonists. Although the lethal effects of glutamate receptor agonists are seen in only a subset of cultured motor neurons, the basis of this selectivity is unlikely to be simply the glutamate receptor phenotype or the level/pattern of rise in agonist-evoked intracellular Ca2+. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/9930721/Excitotoxic_death_of_a_subset_of_embryonic_rat_motor_neurons_in_vitro_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=1999&volume=72&issue=2&spage=500 DB - PRIME DP - Unbound Medicine ER -