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Pilocarpine hydrochloride in ophthalmic solutions: modification of a high-pressure liquid chromatographic determination and survey.
J Assoc Off Anal Chem 1976; 59(6):1409-15JA

Abstract

Pilocarpine undergoes 2 important reactions in solutions, epimerization to isopilocarpine and hydrolysis to pilocarpic acid. There is no official USP limit for isopilocarpine or pilocarpic acid in pilocarpine hydrochloride ophthalmic solutions. An existing high-pressure liquid chromatographic (HPLC) method was modified by changing the buffer system to permit its use with constant-pressure as well as constant-volume instruments. The procedure separates all 3 components on a cation exchange resin; pilocarpine and isopilocarpine are determined directly and pilocarpic acid indirectly. Ophthalmic solutions and drug substances were obtained from substantially all the United States marketers of pilocarpine opthalmic solutions and were analyzed by the modified HPLC method. Results of the survey show that isopilocarpine is present to the extent of 0.4-3% and pilocarpic acid at levels of 0.6-7% of total alkaloid.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

993193

Citation

Weber, J D.. "Pilocarpine Hydrochloride in Ophthalmic Solutions: Modification of a High-pressure Liquid Chromatographic Determination and Survey." Journal - Association of Official Analytical Chemists, vol. 59, no. 6, 1976, pp. 1409-15.
Weber JD. Pilocarpine hydrochloride in ophthalmic solutions: modification of a high-pressure liquid chromatographic determination and survey. J Assoc Off Anal Chem. 1976;59(6):1409-15.
Weber, J. D. (1976). Pilocarpine hydrochloride in ophthalmic solutions: modification of a high-pressure liquid chromatographic determination and survey. Journal - Association of Official Analytical Chemists, 59(6), pp. 1409-15.
Weber JD. Pilocarpine Hydrochloride in Ophthalmic Solutions: Modification of a High-pressure Liquid Chromatographic Determination and Survey. J Assoc Off Anal Chem. 1976;59(6):1409-15. PubMed PMID: 993193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pilocarpine hydrochloride in ophthalmic solutions: modification of a high-pressure liquid chromatographic determination and survey. A1 - Weber,J D, PY - 1976/11/1/pubmed PY - 1976/11/1/medline PY - 1976/11/1/entrez SP - 1409 EP - 15 JF - Journal - Association of Official Analytical Chemists JO - J Assoc Off Anal Chem VL - 59 IS - 6 N2 - Pilocarpine undergoes 2 important reactions in solutions, epimerization to isopilocarpine and hydrolysis to pilocarpic acid. There is no official USP limit for isopilocarpine or pilocarpic acid in pilocarpine hydrochloride ophthalmic solutions. An existing high-pressure liquid chromatographic (HPLC) method was modified by changing the buffer system to permit its use with constant-pressure as well as constant-volume instruments. The procedure separates all 3 components on a cation exchange resin; pilocarpine and isopilocarpine are determined directly and pilocarpic acid indirectly. Ophthalmic solutions and drug substances were obtained from substantially all the United States marketers of pilocarpine opthalmic solutions and were analyzed by the modified HPLC method. Results of the survey show that isopilocarpine is present to the extent of 0.4-3% and pilocarpic acid at levels of 0.6-7% of total alkaloid. SN - 0004-5756 UR - https://www.unboundmedicine.com/medline/citation/993193/Pilocarpine_hydrochloride_in_ophthalmic_solutions:_modification_of_a_high_pressure_liquid_chromatographic_determination_and_survey_ DB - PRIME DP - Unbound Medicine ER -