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Molecular analysis of the APC gene in 205 families: extended genotype-phenotype correlations in FAP and evidence for the role of APC amino acid changes in colorectal cancer predisposition.
J Med Genet. 1999 Jan; 36(1):14-20.JM

Abstract

BACKGROUND/AIMS

The development of colorectal cancer and a variable range of extracolonic manifestations in familial adenomatous polyposis (FAP) is the result of the dominant inheritance of adenomatous polyposis coli (APC) gene mutations. In this study, direct mutation analysis of the APC gene was performed to determine genotype-phenotype correlations for nine extracolonic manifestations and to investigate the incidence of APC mutations in non-FAP colorectal cancer.

METHODS

The APC gene was analysed in 190 unrelated FAP and 15 non-FAP colorectal cancer patients using denaturing gradient gel electrophoresis, the protein truncation test, and direct sequencing.

RESULTS

Chain terminating signals were only identified in patients belonging to the FAP group (105 patients). Amino acid changes were identified in four patients, three of whom belonged to the non-FAP group of colorectal cancer patients. Genotype-phenotype correlations identified significant differences in the nature of certain extracolonic manifestations in FAP patients belonging to three mutation subgroups.

CONCLUSIONS

Extended genotype-phenotype correlations made in this study may have the potential to determine the most appropriate surveillance and prophylactic treatment regimens for those patients with mutations associated with life threatening conditions. This study also provided evidence for the pathological nature of amino acid changes in APC associated with both FAP and non-FAP colorectal cancer patients.

Authors+Show Affiliations

DNA Laboratory, Birmingham Heartlands Hospital, Bordesley Green, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9950360

Citation

Wallis, Y L., et al. "Molecular Analysis of the APC Gene in 205 Families: Extended Genotype-phenotype Correlations in FAP and Evidence for the Role of APC Amino Acid Changes in Colorectal Cancer Predisposition." Journal of Medical Genetics, vol. 36, no. 1, 1999, pp. 14-20.
Wallis YL, Morton DG, McKeown CM, et al. Molecular analysis of the APC gene in 205 families: extended genotype-phenotype correlations in FAP and evidence for the role of APC amino acid changes in colorectal cancer predisposition. J Med Genet. 1999;36(1):14-20.
Wallis, Y. L., Morton, D. G., McKeown, C. M., & Macdonald, F. (1999). Molecular analysis of the APC gene in 205 families: extended genotype-phenotype correlations in FAP and evidence for the role of APC amino acid changes in colorectal cancer predisposition. Journal of Medical Genetics, 36(1), 14-20.
Wallis YL, et al. Molecular Analysis of the APC Gene in 205 Families: Extended Genotype-phenotype Correlations in FAP and Evidence for the Role of APC Amino Acid Changes in Colorectal Cancer Predisposition. J Med Genet. 1999;36(1):14-20. PubMed PMID: 9950360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular analysis of the APC gene in 205 families: extended genotype-phenotype correlations in FAP and evidence for the role of APC amino acid changes in colorectal cancer predisposition. AU - Wallis,Y L, AU - Morton,D G, AU - McKeown,C M, AU - Macdonald,F, PY - 1999/2/9/pubmed PY - 1999/2/9/medline PY - 1999/2/9/entrez SP - 14 EP - 20 JF - Journal of medical genetics JO - J. Med. Genet. VL - 36 IS - 1 N2 - BACKGROUND/AIMS: The development of colorectal cancer and a variable range of extracolonic manifestations in familial adenomatous polyposis (FAP) is the result of the dominant inheritance of adenomatous polyposis coli (APC) gene mutations. In this study, direct mutation analysis of the APC gene was performed to determine genotype-phenotype correlations for nine extracolonic manifestations and to investigate the incidence of APC mutations in non-FAP colorectal cancer. METHODS: The APC gene was analysed in 190 unrelated FAP and 15 non-FAP colorectal cancer patients using denaturing gradient gel electrophoresis, the protein truncation test, and direct sequencing. RESULTS: Chain terminating signals were only identified in patients belonging to the FAP group (105 patients). Amino acid changes were identified in four patients, three of whom belonged to the non-FAP group of colorectal cancer patients. Genotype-phenotype correlations identified significant differences in the nature of certain extracolonic manifestations in FAP patients belonging to three mutation subgroups. CONCLUSIONS: Extended genotype-phenotype correlations made in this study may have the potential to determine the most appropriate surveillance and prophylactic treatment regimens for those patients with mutations associated with life threatening conditions. This study also provided evidence for the pathological nature of amino acid changes in APC associated with both FAP and non-FAP colorectal cancer patients. SN - 0022-2593 UR - https://www.unboundmedicine.com/medline/citation/9950360/Molecular_analysis_of_the_APC_gene_in_205_families:_extended_genotype_phenotype_correlations_in_FAP_and_evidence_for_the_role_of_APC_amino_acid_changes_in_colorectal_cancer_predisposition_ L2 - http://jmg.bmj.com/cgi/pmidlookup?view=long&pmid=9950360 DB - PRIME DP - Unbound Medicine ER -