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Myogenic determination occurs independently in somites and limb buds.
Dev Biol. 1999 Feb 15; 206(2):219-31.DB

Abstract

Gene targeting has indicated that the bHLH transcription factors Myf-5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are entirely ablated in mouse embryos lacking both Myf-5 and MyoD. Entrance into the skeletal myogenic program during development occurs following the independent transcriptional induction of either Myf-5 or MyoD. To identify sequences required for the de novo induction of MyoD transcription during development, we investigated the expression patterns of MyoD-lacZ transgenes in embryos deficient in both Myf-5 and MyoD. We observed that a 258-bp fragment containing the core of the -20-kb MyoD enhancer activated expression in newly formed somites and limb buds in compound mutant embryos lacking both Myf-5 and MyoD. Importantly, Myf-5- and MyoD-deficient presumptive muscle precursor cells expressing beta-galactosidase were observed to assume nonmuscle fates primarily as precartilage primordia in the trunk and the limbs, suggesting that these cells were multipotential. Therefore, cells are recruited into the MyoD-dependent myogenic lineage through activation of the -20-kb MyoD enhancer and this occurs independently in somites and limb buds.

Authors+Show Affiliations

Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9986734

Citation

Kablar, B, et al. "Myogenic Determination Occurs Independently in Somites and Limb Buds." Developmental Biology, vol. 206, no. 2, 1999, pp. 219-31.
Kablar B, Krastel K, Ying C, et al. Myogenic determination occurs independently in somites and limb buds. Dev Biol. 1999;206(2):219-31.
Kablar, B., Krastel, K., Ying, C., Tapscott, S. J., Goldhamer, D. J., & Rudnicki, M. A. (1999). Myogenic determination occurs independently in somites and limb buds. Developmental Biology, 206(2), 219-31.
Kablar B, et al. Myogenic Determination Occurs Independently in Somites and Limb Buds. Dev Biol. 1999 Feb 15;206(2):219-31. PubMed PMID: 9986734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myogenic determination occurs independently in somites and limb buds. AU - Kablar,B, AU - Krastel,K, AU - Ying,C, AU - Tapscott,S J, AU - Goldhamer,D J, AU - Rudnicki,M A, PY - 1999/2/13/pubmed PY - 1999/2/13/medline PY - 1999/2/13/entrez SP - 219 EP - 31 JF - Developmental biology JO - Dev Biol VL - 206 IS - 2 N2 - Gene targeting has indicated that the bHLH transcription factors Myf-5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are entirely ablated in mouse embryos lacking both Myf-5 and MyoD. Entrance into the skeletal myogenic program during development occurs following the independent transcriptional induction of either Myf-5 or MyoD. To identify sequences required for the de novo induction of MyoD transcription during development, we investigated the expression patterns of MyoD-lacZ transgenes in embryos deficient in both Myf-5 and MyoD. We observed that a 258-bp fragment containing the core of the -20-kb MyoD enhancer activated expression in newly formed somites and limb buds in compound mutant embryos lacking both Myf-5 and MyoD. Importantly, Myf-5- and MyoD-deficient presumptive muscle precursor cells expressing beta-galactosidase were observed to assume nonmuscle fates primarily as precartilage primordia in the trunk and the limbs, suggesting that these cells were multipotential. Therefore, cells are recruited into the MyoD-dependent myogenic lineage through activation of the -20-kb MyoD enhancer and this occurs independently in somites and limb buds. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/9986734/Myogenic_determination_occurs_independently_in_somites_and_limb_buds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(98)99126-6 DB - PRIME DP - Unbound Medicine ER -