Attitudes regarding flight deck automation were surveyed in a sample of 5,879 airline pilots from 12 nations. The average difference in endorsement levels across 11 items for pilots flying automated aircraft in 12 nations was 53%, reflecting significant national differences in attitudes on all items, with the largest differences observed for preference and enthusiasm for automation. The range of agreement across nations was on average four times larger than the range of agreement across different airlines within the same nation, and roughly six times larger than the range across pilots of standard and pilots of automated aircraft. Patterns of response are described in terms of dimensions of national culture. Implications of the results for development of safety cultures and culturally sensitive training are discussed.