Prevalence of sensitization to Artemisia allergens Art v 1, Art v 3 and Art v 60 kDa. Cross-reactivity among Art v 3 and other relevant lipid-transfer protein allergens.
Clin Exp Allergy. 2004 Sep; 34(9):1415-21.CE

Abstract

BACKGROUND

Artemisia vulgaris is a widespread weed in the Mediterranean area and several allergens have been detected in its pollen. One of them, Art v 3, belongs to the lipid-transfer protein (LTP) family and its prevalence in Artemisia-sensitized patients or its relationship with other LTP allergens is not clear.

OBJECTIVE

To assess the pattern of sensitization to an array of mugwort allergens in a Mediterranean population, and to study the cross-reactivity of Art v 3 with Pru p 3 and Par j 1, relevant LTP allergens in the area.

METHODS

Skin prick test was performed with whole extracts (A. vulgaris, Parietaria judaica and peach) and pure natural allergens Art v 1, Art v 3, Art v 60 kDa and Par j 1 in 24 mugwort-allergic patients from a Mediterranean area. In vitro assays included measurement of specific IgE and ELISA inhibition among LTP allergens.

RESULTS

The three Artemisia allergens elicited a positive skin response in 70-80% of the patients. Seven patients were clearly sensitized to Par j 1 and 11 to Pru p 3. There was no correlation between Par j 1 and Pru p 3 sensitization, but a highly significant correlation was found between peach extract and Art v 3 as regards the skin response. No IgE cross-reactivity was observed between Art v 3/Par j 1 or Pru p 3/Par j 1. In contrast, Art v 3 significantly inhibited the binding to Pru p 3 of IgE from three patients' sera out of six studied, but Pru p 3 was not able to inhibit the IgE binding to Art v 3.

CONCLUSION

Art v 3 is a major mugwort allergen and in some patients with IgE to both Art v 3 and Pru p 3, Art v 3 behaves as the primary sensitizing agent.

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Authors+Show Affiliations

Lombardero M
Dept. I+D, ALK-Abelló SA, Madrid, Spain. manuel.lombardero@es.alk-abello.com
García-Sellés FJ
No affiliation info available
Polo F
No affiliation info available
Jimeno L
No affiliation info available
Chamorro MJ
No affiliation info available
García-Casado G
No affiliation info available
Sánchez-Monge R
No affiliation info available
Díaz-Perales A
No affiliation info available
Salcedo G
No affiliation info available
Barber D
No affiliation info available

MeSH

AllergensAntigens, PlantArtemisiaCarrier ProteinsCross ReactionsEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin EParietariaPlant ExtractsPlant ProteinsPollenRhinitis, Allergic, SeasonalSkin TestsSpain

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15347375