The aim of this study was to investigate an outbreak of enterovirus infection in Lithuania in 2001, as well as the clinical presentation and outcome of the disease. Thirty children aged 1 month to 15 years were referred to hospital with suspected enterovirus infection during the period August-October 2001. Echovirus 13 (EV-13) was isolated from eight (26.7%) cases. No other pathogens were detected. Infection presented predominantly as viral meningitis, and rarely as a febrile illness with rash. The outcome was good for all patients. This is the first report of EV-13 from Lithuania.