To study the chemical constituents of the 95% ethanol extract from the rhizome of Smilax china.
Seven compounds were isolated from ethyl acetate fraction of 95% ethanol extract by using repeated silica column chromatography, partition chromatography, preparing TLC, and other methods. Structure of three compounds of them was identified based on chemical methods and spectroscopic methods.
The structure of them were established as dihydrokaempferol-5-O-beta-D-glucoside (I), beta-sitosterol (II), daucosterol (III).
Compound I was a novel compound which was first found from natural plants.