Inactivation of protein translocation by cold-sensitive mutations in the yajC-secDF operon.
Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation.
Amino Acid SequenceBacterial ProteinsCold TemperatureEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialIntracellular MembranesMembrane ProteinsMembrane Transport ProteinsMolecular Sequence DataMutationOperon
Research Support, Non-U.S. Gov't