The hydrodistilled essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. were analyzed by GC and GC/MS. The flower oil was characterized by high amounts of myristicin (42.5%) and trans-beta-ocimene (41.0%). In the leaf oil eighteen constituents, representing 99.3% of the total, were identified of which trans-beta-ocimene (24.9%), myristicin (15.7%), terpinolene (14.5%), fenchyl acetate (13.9%), cis-beta-ocimene (6.3%) and sabinene (6.1%) were major components. The main components of the stem oil were trans-beta-ocimene (54.2%), myristicin (22.4%) and sabinene (8.9%). Antibacterial activities of the flower, leaf and stem oils were evaluated using the micro-dilution broth method. Inhibitory effects on Escherichia coli and Salmonella typhi were recorded.