Validation of an enzyme-linked immunosorbent assay that detects Histoplasma capsulatum antigenuria in Colombian patients with AIDS for diagnosis and follow-up during therapy.
We validated an antigen capture enzyme-linked immunosorbent assay (ELISA) in Colombian persons with AIDS and proven histoplasmosis and evaluated the correlation between antigenuria and clinical improvement during follow-up. The sensitivity of the Histoplasma capsulatum ELISA was 86%, and the overall specificity was 94%. The antigen test successfully monitored the response to therapy.
AIDS-Related Opportunistic InfectionsAntigens, FungalClinical Laboratory TechniquesCohort StudiesColombiaDrug MonitoringEnzyme-Linked Immunosorbent AssayHistoplasmaHistoplasmosisHumansProspective StudiesSensitivity and Specificity
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.