Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria).
Phytother Res. 2015 Jan; 29(1):48-58.PR

Abstract

Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.

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Authors+Show Affiliations

Massaro CF
Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, Australia.
Smyth TJ
No affiliation info available
Smyth WF
No affiliation info available
Heard T
No affiliation info available
Leonhardt SD
No affiliation info available
Katouli M
No affiliation info available
Wallace HM
No affiliation info available
Brooks P
No affiliation info available

MeSH

AnimalsAnti-Bacterial AgentsAustraliaBeesBiological ProductsFlavonoidsGas Chromatography-Mass SpectrometryMolecular StructurePhloroglucinolPropolisPseudomonas aeruginosaStaphylococcus aureusTerpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25230727