Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria).
Phytother Res 2015; 29(1):48-58PR

Abstract

Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.

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    Authors+Show Affiliations

    Massaro CF
    Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, Australia.
    Smyth TJ
    No affiliation info available
    Smyth WF
    No affiliation info available
    Heard T
    No affiliation info available
    Leonhardt SD
    No affiliation info available
    Katouli M
    No affiliation info available
    Wallace HM
    No affiliation info available
    Brooks P
    No affiliation info available

    MeSH

    AnimalsAnti-Bacterial AgentsAustraliaBeesBiological ProductsFlavonoidsGas Chromatography-Mass SpectrometryMolecular StructurePhloroglucinolPropolisPseudomonas aeruginosaStaphylococcus aureusTerpenes

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    25230727